PXD018706

PXD018706 is an original dataset announced via ProteomeXchange.

Title	Nascent RNA antagonises the interaction of regulatory proteins with chromatin
Description	A number of transcriptional and chromatin regulatory proteins have been reported to be recruited to chromatin by specific regulatory RNAs. Whether RNA has a more general role in the interaction of proteins with chromatin is unknown. We used proteomics methods to determine the global impact of nascent RNA on chromatin in embryonic stem cells. Surprisingly, we found that nascent RNA primarily antagonises the interaction of chromatin modifiers and transcriptional regulators with chromatin. Transcriptional inhibition and RNA degradation induced recruitment of a set of transcriptional regulators, chromatin modifiers, nucleosome remodelers, and regulators of higher-order structure. RNA directly bound to factors including BAF, NuRD, EHMT1 and INO80 and inhibited their interaction with nucleosomes. In the case of the transcriptional elongation factor P-TEFb, direct binding to pre-mRNA released it from the chromatin-associated 7SK ribonucleoprotein complex. We propose that through these mechanisms, nascent RNA provides a direct link between transcriptional output and chromatin state.
HostingRepository	PRIDE
AnnounceDate	2021-06-15
AnnouncementXML	Submission_2021-06-14_23:47:46.275.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Peter Faull
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	iodoacetamide derivatized residue
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2020-04-21 05:33:58	ID requested
⏵ 1	2021-06-14 23:47:46	announced

Dataset with its publication pending

submitter keyword: RNA, chromatin, regulatory proteins

Bram Snijders
contact affiliation	Proteomics Science and Technology Platform, The Francis Crick Institute
contact email	bram.snijders@crick.ac.uk
lab head
Peter Faull
contact affiliation	The Francis Crick Institute
contact email	peter.faull@crick.ac.uk
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD018706
     1. Label: PRIDE project
     2. Name: Nascent RNA antagonises the interaction of regulatory proteins with chromatin