PXD018702 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Characterization of protein dynamics after alpha-amanitin exposure in hepatoma cells |
Description | Alpha-amanitin (α-AMA) is a cyclic peptide and is one of the most lethal mushroom amatoxins found in Amanita phalloides. α-AMA is known to cause hepatotoxicity through RNA polymerase II inhibition, which acts in RNA and DNA translocation. To investigate the toxic signature of α-AMA beyond known mechanisms, we used quantitative nanoflow liquid chromatography-tandem mass spectrometry analysis coupled with tandem mass tag labeling to examine proteome dynamics in Huh-7 human hepatoma cells treated with toxic concentrations of α-AMA. We identified 1,828 proteins, quantified 1,563 proteins, and found a decrease of four subunits in T-complex protein 1-ring complex protein that was dependent on the α-AMA concentration. We conducted bioinformatics analyses of the quantified proteins to characterize the toxic signature of α-AMA in hepatoma cells. This is the first report of global proteome abundance changes according to α-AMA concentration variations and suggests a possible novel molecular regulation mechanism for hepatotoxicity. |
HostingRepository | PRIDE |
AnnounceDate | 2024-10-22 |
AnnouncementXML | Submission_2024-10-22_05:27:27.693.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Doeun Kim |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | No PTMs are included in the dataset |
Instrument | LTQ Orbitrap |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2020-04-21 01:56:39 | ID requested | |
1 | 2021-09-08 18:48:46 | announced | |
⏵ 2 | 2024-10-22 05:27:35 | announced | 2024-10-22: Updated project metadata. |
Publication List
10.3390/toxins13030197; |
Kim D, Kim S, Na AY, Sohn CH, Lee S, Lee HS, Identification of Decrease in TRiC Proteins as Novel Targets of Alpha-Amanitin-Derived Hepatotoxicity by Comparative Proteomic Analysis In Vitro. Toxins (Basel), 13(3):(2021) [pubmed] |
Keyword List
submitter keyword: TRiC,alpha-amanitin, hepatotoxicity, comparative quantitative proteomics |
Contact List
Sangkyu Lee |
contact affiliation | College of Pharmacy and Research Institute of Pharmaceutical Sciences, Kyungpook National University, Daegu 41566, Republic of Korea |
contact email | sangkyu@knu.ac.kr |
lab head | |
Doeun Kim |
contact affiliation | College of Pharmacy and Research Institute of Pharmaceutical Sciences, Kyungpook National University, Daegu 41566, Republic of Korea |
contact email | kdkdl1230@gmail.com |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD018702
- Label: PRIDE project
- Name: Characterization of protein dynamics after alpha-amanitin exposure in hepatoma cells