PXD018699

PXD018699 is an original dataset announced via ProteomeXchange.

Dataset Summary

Title	Extending the identification coverage of hydrophilic proteome and phosphoproteome via a graphite solid phase extraction StageTip
Description	Desalting and concentrating peptides using reverse phase chromatography prior LC-MS/MS analysis is a critical step to improve the sensitivity of peptide detection. However, hydrophilic peptides are not easy to retain on the conventional solid phase extraction (SPE) StageTip using C18 as materials during the desalting step. In this study, graphite carbon was utilized as a SPE StageTip with strong hydrophobic adsorbent, which doubled the identification number of multiply phosphopeptides compared to the number using C18 SPE StageTip. In addition, (phospho)peptides with lower GRAVY value and shorter peptides length were enriched using the graphite SPE StageTip. Finally, a total of 7,617 phosphopeptides were identified, which covers both hydrophilic and hydrophobic properties. The complementary affinity of different desalting materials including C18, SDB-XC, and graphite was further demonstrated to deepen the coverage of proteome and phosphoproteome.
HostingRepository	jPOST
AnnounceDate	2021-04-21
AnnouncementXML	Submission_2022-09-18_02:34:25.802.xml
DigitalObjectIdentifier
ReviewLevel	Non peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Chuan-Chih Hsu
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606; scientific name: Arabidopsis thaliana (Mouse-ear cress); NCBI TaxID: 3702;
ModificationList	S-carboxamidomethyl-L-cysteine; L-methionine sulfoxide; O-phospho-L-serine; O-phospho-L-threonine; O4'-phospho-L-tyrosine
Instrument	LTQ Orbitrap Velos

Dataset History

Revision	Datetime	Status	ChangeLog Entry
0	2020-04-20 13:47:43	ID requested
1	2021-04-20 08:00:05	announced
⏵ 2	2022-09-18 02:34:26	announced	2022-09-18: Updated FTP location.

Publication List

Dataset with its publication pending

Dataset with its publication pending

Keyword List

submitter keyword: solid phase extraction, graphite SPE StageTip, hydrophilic peptide

submitter keyword: solid phase extraction, graphite SPE StageTip, hydrophilic peptide

Contact List

Andy W. Tao
lab head
Chuan-Chih Hsu
contact affiliation	Purdue University
dataset submitter

Full Dataset Link List

jPOST dataset URI
Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.jpostdb.org/JPST000833/

jPOST dataset URI

Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.jpostdb.org/JPST000833/