PXD018691 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Truncated PARP1 promotes RNA Polymerase III during apoptosis |
Description | The N-terminal three zinc finger motifs of PARP1 are evolutionarily conserved in multicellular organism。However, during apoptosis, human PARP1 is mainly cleaved by caspase 3 at D214. As a result, the truncated PARP1 loses two N-terminal zinc finger motifs and only contains the third zinc finger motif, the BRCT domain, the WGR domain and the C-terminal catalytic domain. Interestingly, when we explored the domain architecture of PARP1 in other organisms, we found that similar to human tPARP1, PARP1 orthologs in several lower organisms do not have the N-terminal two zinc fingers or even they lack the third zinc finger motif. It indicates that even without the first two zinc finger motifs, tPARP1 may still catalyze ADP-ribosylation and play an important role in certain biological processes. To reveal the biological function of tPARP1, we performed tandem affinity purification and searched for the possible substrates. |
HostingRepository | PRIDE |
AnnounceDate | 2024-10-22 |
AnnouncementXML | Submission_2024-10-22_05:31:48.390.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Qian Chen |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | No PTMs are included in the dataset |
Instrument | LTQ Orbitrap |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2020-04-20 08:47:36 | ID requested | |
1 | 2022-02-15 11:02:06 | announced | |
⏵ 2 | 2024-10-22 05:31:56 | announced | 2024-10-22: Updated project metadata. |
Publication List
10.1038/s41421-021-00355-1; |
Chen Q, Ma K, Liu X, Chen SH, Li P, Yu Y, Leung AKL, Yu X, Truncated PARP1 mediates ADP-ribosylation of RNA polymerase III for apoptosis. Cell Discov, 8(1):3(2022) [pubmed] |
Keyword List
submitter keyword: Turncated PARP1, LC-MS/MS |
Contact List
Qian Chen |
contact affiliation | Department of Cancer Genetics and Epigenetics, Beckman Research Institute, City of Hope Medical Center, Duarte, CA 91010, USA |
contact email | qchen@coh.org |
lab head | |
Qian Chen |
contact affiliation | City of hope |
contact email | qchen@coh.org |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD018691
- Label: PRIDE project
- Name: Truncated PARP1 promotes RNA Polymerase III during apoptosis