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PXD018691

PXD018691 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleTruncated PARP1 promotes RNA Polymerase III during apoptosis
DescriptionThe N-terminal three zinc finger motifs of PARP1 are evolutionarily conserved in multicellular organism。However, during apoptosis, human PARP1 is mainly cleaved by caspase 3 at D214. As a result, the truncated PARP1 loses two N-terminal zinc finger motifs and only contains the third zinc finger motif, the BRCT domain, the WGR domain and the C-terminal catalytic domain. Interestingly, when we explored the domain architecture of PARP1 in other organisms, we found that similar to human tPARP1, PARP1 orthologs in several lower organisms do not have the N-terminal two zinc fingers or even they lack the third zinc finger motif. It indicates that even without the first two zinc finger motifs, tPARP1 may still catalyze ADP-ribosylation and play an important role in certain biological processes. To reveal the biological function of tPARP1, we performed tandem affinity purification and searched for the possible substrates.
HostingRepositoryPRIDE
AnnounceDate2024-10-22
AnnouncementXMLSubmission_2024-10-22_05:31:48.390.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterQian Chen
SpeciesList scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationListNo PTMs are included in the dataset
InstrumentLTQ Orbitrap
Dataset History
RevisionDatetimeStatusChangeLog Entry
02020-04-20 08:47:36ID requested
12022-02-15 11:02:06announced
22024-10-22 05:31:56announced2024-10-22: Updated project metadata.
Publication List
10.1038/s41421-021-00355-1;
Chen Q, Ma K, Liu X, Chen SH, Li P, Yu Y, Leung AKL, Yu X, Truncated PARP1 mediates ADP-ribosylation of RNA polymerase III for apoptosis. Cell Discov, 8(1):3(2022) [pubmed]
Keyword List
submitter keyword: Turncated PARP1, LC-MS/MS
Contact List
Qian Chen
contact affiliationDepartment of Cancer Genetics and Epigenetics, Beckman Research Institute, City of Hope Medical Center, Duarte, CA 91010, USA
contact emailqchen@coh.org
lab head
Qian Chen
contact affiliationCity of hope
contact emailqchen@coh.org
dataset submitter
Full Dataset Link List
Dataset FTP location
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PRIDE project URI
Repository Record List
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