PXD018691

PXD018691 is an original dataset announced via ProteomeXchange.

Title	Truncated PARP1 promotes RNA Polymerase III during apoptosis
Description	The N-terminal three zinc finger motifs of PARP1 are evolutionarily conserved in multicellular organism。However, during apoptosis, human PARP1 is mainly cleaved by caspase 3 at D214. As a result, the truncated PARP1 loses two N-terminal zinc finger motifs and only contains the third zinc finger motif, the BRCT domain, the WGR domain and the C-terminal catalytic domain. Interestingly, when we explored the domain architecture of PARP1 in other organisms, we found that similar to human tPARP1, PARP1 orthologs in several lower organisms do not have the N-terminal two zinc fingers or even they lack the third zinc finger motif. It indicates that even without the first two zinc finger motifs, tPARP1 may still catalyze ADP-ribosylation and play an important role in certain biological processes. To reveal the biological function of tPARP1, we performed tandem affinity purification and searched for the possible substrates.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_05:31:48.390.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Qian Chen
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	LTQ Orbitrap

Revision	Datetime	Status	ChangeLog Entry
0	2020-04-20 08:47:36	ID requested
1	2022-02-15 11:02:06	announced
⏵ 2	2024-10-22 05:31:56	announced	2024-10-22: Updated project metadata.

10.1038/s41421-021-00355-1;

Chen Q, Ma K, Liu X, Chen SH, Li P, Yu Y, Leung AKL, Yu X, Truncated PARP1 mediates ADP-ribosylation of RNA polymerase III for apoptosis. Cell Discov, 8(1):3(2022) [pubmed]

submitter keyword: Turncated PARP1, LC-MS/MS

Qian Chen
contact affiliation	Department of Cancer Genetics and Epigenetics, Beckman Research Institute, City of Hope Medical Center, Duarte, CA 91010, USA
contact email	qchen@coh.org
lab head
Qian Chen
contact affiliation	City of hope
contact email	qchen@coh.org
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/02/PXD018691

PRIDE project URI

• PRIDE
  1. PXD018691
     1. Label: PRIDE project
     2. Name: Truncated PARP1 promotes RNA Polymerase III during apoptosis