<<< Full experiment listing

PXD018672

PXD018672 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleMolecular connection between the TUTase URT1 and decapping activators
DescriptionUridylation is a widespread modification destabilizing eukaryotic mRNAs. Yet, molecular mechanisms underlying TUTase-mediated mRNA degradation remain mostly unresolved. Here, we report that the Arabidopsis TUTase URT1 participates in a molecular network connecting several translational repressors/decapping activators including DECAPPING 5 (DCP5), the Arabidopsis ortholog of human LSM14 and yeast Scd6. A conserved Helical Leucine-rich Motif (HLM) within an intrinsically disordered region of URT1 binds to the LSm domain of DCP5. This interaction connects URT1 to additional decay factors like DDX6/Dhh1-like RNA helicases. The combination of in planta and in vitro analyses supports a model that explains how URT1 reduces the accumulation of oligo(A)-tailed mRNAs: first, by connecting decapping factors and second, because 3’ terminal uridines can intrinsically hinder deadenylation. Importantly, preventing the accumulation of excessively deadenylated mRNAs in Arabidopsis avoids the biogenesis of illegitimate siRNAs that silence endogenous mRNAs and perturb plant growth and development.
HostingRepositoryPRIDE
AnnounceDate2024-10-08
AnnouncementXMLSubmission_2024-10-08_11:17:25.759.xml
DigitalObjectIdentifierhttps://dx.doi.org/10.6019/PXD018672
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportSupported dataset by repository
PrimarySubmitterLauriane Kuhn
SpeciesList scientific name: Arabidopsis thaliana (Mouse-ear cress); NCBI TaxID: 3702;
ModificationListGG; Phospho; Oxidation; Acetyl; Carbamidomethyl
InstrumentTripleTOF 5600; Q Exactive
Dataset History
RevisionDatetimeStatusChangeLog Entry
02020-04-20 00:56:28ID requested
12024-10-08 11:17:26announced
Publication List
10.6019/PXD018672;
Keyword List
submitter keyword: Arabidopsis, TUTase, deadenylation, RNA degradation, decapping,Uridylation
Contact List
Hélène Zuber
contact affiliationInstitut de biologie moléculaire des plantes, CNRS, Université de Strasbourg, 12 rue Zimmer, 67000 Strasbourg, France
contact emailhelene.zuber@ibmp-cnrs.unistra.fr
lab head
Lauriane Kuhn
contact affiliationCNRS, IBMC, FRC1589
contact emaill.kuhn@ibmc-cnrs.unistra.fr
dataset submitter
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2020/05/PXD018672
PRIDE project URI
Repository Record List
[ + ]