PXD018672

PXD018672 is an original dataset announced via ProteomeXchange.

Title	Molecular connection between the TUTase URT1 and decapping activators
Description	Uridylation is a widespread modification destabilizing eukaryotic mRNAs. Yet, molecular mechanisms underlying TUTase-mediated mRNA degradation remain mostly unresolved. Here, we report that the Arabidopsis TUTase URT1 participates in a molecular network connecting several translational repressors/decapping activators including DECAPPING 5 (DCP5), the Arabidopsis ortholog of human LSM14 and yeast Scd6. A conserved Helical Leucine-rich Motif (HLM) within an intrinsically disordered region of URT1 binds to the LSm domain of DCP5. This interaction connects URT1 to additional decay factors like DDX6/Dhh1-like RNA helicases. The combination of in planta and in vitro analyses supports a model that explains how URT1 reduces the accumulation of oligo(A)-tailed mRNAs: first, by connecting decapping factors and second, because 3’ terminal uridines can intrinsically hinder deadenylation. Importantly, preventing the accumulation of excessively deadenylated mRNAs in Arabidopsis avoids the biogenesis of illegitimate siRNAs that silence endogenous mRNAs and perturb plant growth and development.
HostingRepository	PRIDE
AnnounceDate	2024-10-08
AnnouncementXML	Submission_2024-10-08_11:17:25.759.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD018672
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Lauriane Kuhn
SpeciesList	scientific name: Arabidopsis thaliana (Mouse-ear cress); NCBI TaxID: 3702;
ModificationList	GG; Phospho; Oxidation; Acetyl; Carbamidomethyl
Instrument	TripleTOF 5600; Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2020-04-20 00:56:28	ID requested
⏵ 1	2024-10-08 11:17:26	announced

10.6019/PXD018672;

submitter keyword: Arabidopsis, TUTase, deadenylation, RNA degradation, decapping,Uridylation

Hélène Zuber
contact affiliation	Institut de biologie moléculaire des plantes, CNRS, Université de Strasbourg, 12 rue Zimmer, 67000 Strasbourg, France
contact email	helene.zuber@ibmp-cnrs.unistra.fr
lab head
Lauriane Kuhn
contact affiliation	CNRS, IBMC, FRC1589
contact email	l.kuhn@ibmc-cnrs.unistra.fr
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2020/05/PXD018672

PRIDE project URI

• PRIDE
  1. PXD018672
     1. Label: PRIDE project
     2. Name: Molecular connection between the TUTase URT1 and decapping activators