PXD018663

PXD018663 is an original dataset announced via ProteomeXchange.

Dataset Summary

Title	Fractionation of phosphopeptides using reversed phase microcolumn prior to LC-MS analysis
Description	A protocol for an improved phosphopeptide identification in tryptically digested complex peptide samples is described. The common TiO2 based phoshopeptide enrichment is coupled to a simple peptide fractionation protocol with following LC-MS analysis of the obtained fractions and proteomic identification of phosphorylated peptides. The fractions are obtained by an optimized protocol for peptide fractionation using a home-made capillary column coupled to a microgradient elution using a gastight microsyringe. The protocol leads to a substantially increased number of phosphopeptides (more than twice).
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_05:11:38.732.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Pavel Rehulka
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	phosphorylated residue
Instrument	Q Exactive

Dataset History

Revision	Datetime	Status	ChangeLog Entry
0	2020-04-19 10:05:32	ID requested
1	2020-08-31 00:35:57	announced
⏵ 2	2024-10-22 05:11:44	announced	2024-10-22: Updated project metadata.

Publication List

10.3390/ijms21113971;
Ondrej M, Rehulka P, Rehulkova H, Kupcik R, Tichy A, Fractionation of Enriched Phosphopeptides Using pH/Acetonitrile-Gradient-Reversed-Phase Microcolumn Separation in Combination with LC-MS/MS Analysis. Int J Mol Sci, 21(11):(2020) [pubmed]

10.3390/ijms21113971;

Ondrej M, Rehulka P, Rehulkova H, Kupcik R, Tichy A, Fractionation of Enriched Phosphopeptides Using pH/Acetonitrile-Gradient-Reversed-Phase Microcolumn Separation in Combination with LC-MS/MS Analysis. Int J Mol Sci, 21(11):(2020) [pubmed]

Keyword List

submitter keyword: phosphopeptide fractionation, H1299 cell line,phosphorylation

submitter keyword: phosphopeptide fractionation, H1299 cell line,phosphorylation

Contact List

Jiri Stulik
contact affiliation	Department of Molecular Pathology and Biology Faculty of Military Health Sciences, University of Defence Trebesska 1575, 50001 Hradec Kralove, Czech Republic
contact email	jiri.stulik@unob.cz
lab head
Pavel Rehulka
contact affiliation	Department of Molecular Pathology and Biology FMHS UD, Trebesska 1575, CZ-50001 Hradec Kralove, Czech Republic
contact email	pavel.rehulka@unob.cz
dataset submitter

Full Dataset Link List

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2020/08/PXD018663
PRIDE project URI

Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2020/08/PXD018663

PRIDE project URI

Repository Record List

[ + ]