PXD018662

PXD018662 is an original dataset announced via ProteomeXchange.

Title	USP9X controls the resolution of stalled ribosomes
Description	When a ribosome stalls during translation, it runs the risk of collision with a trailing ribosome. Such an encounter leads to the formation of a stable di-ribosome complex, which needs to be resolved by a dedicated machinery. The initial stalling and the subsequent resolution of di-ribosomal complexes requires activity of Makorin and ZNF598 ubiquitin E3 ligases respectively, through ubiquitylation of the eS10 and uS10 sub-units of the ribosome. It is common for the stability of RING E3 ligases to be regulated by an interacting deubiquitylase, which often opposes auto-ubiquitylation of the E3. Here, we show that the deubiquitylase USP9X directly interacts with ZNF598 and regulates its abundance through the control of protein stability. We have developed a highly specific small molecule inhibitor of USP9X. In the absence of USP9X or following chemical inhibition of its catalytic activity, steady state levels of Makorins and ZNF598 are diminished and the ribosomal quality control pathway is impaired.
HostingRepository	PRIDE
AnnounceDate	2021-01-26
AnnouncementXML	Submission_2021-01-25_22:38:08.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Adan Pinto-Fernandez
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2020-04-19 09:55:36	ID requested
⏵ 1	2021-01-25 22:38:09	announced

Dataset with its publication pending

submitter keyword: ubiquitin, deubiquitylase, USP9X, ribosomal stalling, DUB inhibitor, translation, ZNF598, Makorin

Prof. Benedikt Kessler
contact affiliation	Target Discovery Institute - Nuffield Department of medicine (University of Oxford)
contact email	benedikt.kessler@ndm.ox.ac.uk
lab head
Adan Pinto-Fernandez
contact affiliation	University of Oxford
contact email	adan.pintofernandez@ndm.ox.ac.uk
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/01/PXD018662

PRIDE project URI

• PRIDE
  1. PXD018662
     1. Label: PRIDE project
     2. Name: USP9X controls the resolution of stalled ribosomes