PXD018661

PXD018661 is an original dataset announced via ProteomeXchange.

Title	Olaparib based photo-affinity probes for PARP-1 detection in living cells
Description	The poly-ADP-ribose polymerase (PARP) is a protein from the family of ADP-ribosyltransferases that catalyzes poly adenosine diphosphate ribose (ADPR) formation in order to attract the DNA repair machinery to DNA damage sites. Inhibition of PARP activity by olaparib can cause cell death which is of clinical relevance in some tumor types. This demonstrates that quantification of PARP activity in the context of living cells is of great importance. In this work we present the design, synthesis and biological evaluation of photo-activatable affinity probes inspired by the olaparib molecule which are equipped with a diazirine for covalent attachment upon activation by UV light and a ligation handle for the addition of a reporter group of choice. SDS-PAGE, western blotting and label-free LC-MS/MS quantification analysis show that the probes target the PARP-1 protein and are selectively outcompeted by olaparib suggesting binding in the same enzymatic pocket.
HostingRepository	PRIDE
AnnounceDate	2020-04-29
AnnouncementXML	Submission_2020-04-29_02:48:19.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Bogdan Florea
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Synapt MS

Revision	Datetime	Status	ChangeLog Entry
0	2020-04-19 09:54:18	ID requested
⏵ 1	2020-04-29 02:48:20	announced

Voorneveld J, Florea BI, Bakkum T, Mendowicz RJ, van der Veer MS, Gagestein B, van Kasteren SI, van der Stelt M, Overkleeft HS, Filippov DV, Olaparib-Based Photoaffinity Probes for PARP-1 Detection in Living Cells. Chembiochem, 21(17):2431-2434(2020) [pubmed]

submitter keyword: Olaparib

PARP-1

bioorthogonal chemistry

chemical proteomics

photoaffinity probes

Bogdan Iulius Florea
contact affiliation	dept of Bioorganic synthesis, Leiden Institute for Chemistry, Leiden University, The Netherlands
contact email	b.florea@chem.leidenuniv.nl
lab head
Bogdan Florea
contact affiliation	Leiden Institute for Chemistry
contact email	b.florea@chem.leidenuniv.nl
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD018661
     1. Label: PRIDE project
     2. Name: Olaparib based photo-affinity probes for PARP-1 detection in living cells