PXD018650
PXD018650 is an original dataset announced via ProteomeXchange.
Dataset Summary
Title | Extending the Separation Space with Trapped Ion Mobility Spectrometry Improves the Accuracy of Isobaric Tag-based Quantitation in Proteomic LC/MS/MS |
Description | Two-dimensional separation by nano-LC and trapped ion mobility spectrometry (TIMS) prior to Q-TOF tandem mass spectrometry significantly improves the accuracy of isobaric tag-based quantitation in proteome analysis without the need for additional measurement time for TIMS insertion. The obtained peak capacity of up to 3,300 per hour in LC/TIMS reduced the co-isolation of precursor ions at the quadrupole analyzer, resulting in more accurate ratios of reporter ions derived from isobaric tags in product ion spectra obtained at the TOF analyzer. We also found that TIMS with a narrow quadrupole isolation window could reduce the ratio compression effect at least as effectively as the synchronous precursor selection method using MS3 scans without compromising sensitivity or coverage. Our results suggest that the short-gradient LC/TIMS/Q/TOF system is an excellent platform for high-throughput proteomics studies. |
HostingRepository | jPOST |
AnnounceDate | 2020-05-22 |
AnnouncementXML | Submission_2022-09-18_02:19:10.737.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Kosuke Ogata |
SpeciesList | scientific name: cellular organisms; NCBI TaxID: 131567; |
ModificationList | S-carboxymethyl-L-cysteine; L-methionine sulfoxide; TMT6plex reporter+balance reagent N-acylated residue; TMT6plex reporter+balance reagent acylated N-terminal |
Instrument | instrument; instrument |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
---|---|---|---|
0 | 2020-04-19 04:00:03 | ID requested | |
1 | 2020-06-04 19:45:51 | announced | |
2 | 2021-02-04 17:12:29 | announced | 2021-02-04: Updated FTP location. |
⏵ 3 | 2022-09-18 02:19:11 | announced | 2022-09-18: Updated FTP location. |
Publication List
Ogata K, Ishihama Y, Extending the Separation Space with Trapped Ion Mobility Spectrometry Improves the Accuracy of Isobaric Tag-Based Quantitation in Proteomic LC/MS/MS. Anal Chem, 92(12):8037-8040(2020) [pubmed] |
Keyword List
submitter keyword: Trapped ion mobility spectrometry, Tandem mass tag, Ratio compression, High accuracy quantitation |
Contact List
Yasushi Ishihama | |
---|---|
lab head | |
Kosuke Ogata | |
contact affiliation | Kyoto university |
dataset submitter |
Full Dataset Link List
jPOST dataset URI |
Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.jpostdb.org/JPST000832/ |