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PXD018650

PXD018650 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleExtending the Separation Space with Trapped Ion Mobility Spectrometry Improves the Accuracy of Isobaric Tag-based Quantitation in Proteomic LC/MS/MS
DescriptionTwo-dimensional separation by nano-LC and trapped ion mobility spectrometry (TIMS) prior to Q-TOF tandem mass spectrometry significantly improves the accuracy of isobaric tag-based quantitation in proteome analysis without the need for additional measurement time for TIMS insertion. The obtained peak capacity of up to 3,300 per hour in LC/TIMS reduced the co-isolation of precursor ions at the quadrupole analyzer, resulting in more accurate ratios of reporter ions derived from isobaric tags in product ion spectra obtained at the TOF analyzer. We also found that TIMS with a narrow quadrupole isolation window could reduce the ratio compression effect at least as effectively as the synchronous precursor selection method using MS3 scans without compromising sensitivity or coverage. Our results suggest that the short-gradient LC/TIMS/Q/TOF system is an excellent platform for high-throughput proteomics studies.
HostingRepositoryjPOST
AnnounceDate2020-05-22
AnnouncementXMLSubmission_2022-09-18_02:19:10.737.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterKosuke Ogata
SpeciesList scientific name: cellular organisms; NCBI TaxID: 131567;
ModificationListS-carboxymethyl-L-cysteine; L-methionine sulfoxide; TMT6plex reporter+balance reagent N-acylated residue; TMT6plex reporter+balance reagent acylated N-terminal
Instrumentinstrument; instrument
Dataset History
RevisionDatetimeStatusChangeLog Entry
02020-04-19 04:00:03ID requested
12020-06-04 19:45:51announced
22021-02-04 17:12:29announced2021-02-04: Updated FTP location.
32022-09-18 02:19:11announced2022-09-18: Updated FTP location.
Publication List
Ogata K, Ishihama Y, Extending the Separation Space with Trapped Ion Mobility Spectrometry Improves the Accuracy of Isobaric Tag-Based Quantitation in Proteomic LC/MS/MS. Anal Chem, 92(12):8037-8040(2020) [pubmed]
Keyword List
submitter keyword: Trapped ion mobility spectrometry, Tandem mass tag, Ratio compression, High accuracy quantitation
Contact List
Yasushi Ishihama
lab head
Kosuke Ogata
contact affiliationKyoto university
dataset submitter
Full Dataset Link List
jPOST dataset URI
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.jpostdb.org/JPST000832/