PXD018639

PXD018639 is an original dataset announced via ProteomeXchange.

Title	Analysis of proteomic changes in Fbxl4 knock-out mouse liver and patient fibroblasts
Description	We analyzed the proteomic changes that occur in a Fbxl4 knock-out 1-year mouse. We analyzed Liver tissue using a 6-plex TMT approach (3 KO and 3 controls). Since we observed a global decrease in mitochondrial proteins, we also explored mitoproteome changes in different tissues (liver, kidney and heart) using a label-free approach. Finally, we also did a 6-plex TMT to analize the proteomic changes in 3 patient-derived fibroblast lines compared to 3 control lines and correlated them with the results obtained in the mouse model. All together, these experiments revealed that Fbxl4 deficiency leads to a decreased mitochondrial content without major changes in mitochondria itself, pointing to an increased turnover.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_05:07:03.183.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	David Alsina
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090; scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	TMT6plex-126 reporter+balance reagent acylated residue
Instrument	Q Exactive HF; Orbitrap Fusion

Revision	Datetime	Status	ChangeLog Entry
0	2020-04-18 15:31:57	ID requested
1	2020-06-10 04:46:32	announced
2	2020-06-16 23:00:39	announced	2020-06-17: Updated publication reference for PubMed record(s): 32525278.
⏵ 3	2024-10-22 05:07:05	announced	2024-10-22: Updated project metadata.

10.15252/emmm.201911659;

Alsina D, Lytovchenko O, Schab A, Atanassov I, Schober FA, Jiang M, Koolmeister C, Wedell A, Taylor RW, Wredenberg A, Larsson NG, FBXL4 deficiency increases mitochondrial removal by autophagy. EMBO Mol Med, 12(7):e11659(2020) [pubmed]

submitter keyword: Mitochondria, Human, Mouse, Fibroblast,Fbxl4, Liver

Nils-Göran Larsson
contact affiliation	Medical Biochemistry and Biophysics department, Karolinska Institutet, Stockholm, Sweden
contact email	nils-goran.larsson@ki.se
lab head
David Alsina
contact affiliation	Karolinska Institutet
contact email	david.alsina@ki.se
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2020/06/PXD018639

PRIDE project URI

• PRIDE
  1. PXD018639
     1. Label: PRIDE project
     2. Name: Analysis of proteomic changes in Fbxl4 knock-out mouse liver and patient fibroblasts