PXD018623

PXD018623 is an original dataset announced via ProteomeXchange.

Title	USP7 and VCPFAF1 define the SUMO/Ubiquitin landscape at DNA replication forks
Description	The AAA+ ATPase VCP regulates the extraction of SUMO and/or ubiquitin modified factors from chromatin. We previously showed that active DNA synthesis is associated with a SUMO-high and ubiquitin-low environment that is maintained by the deubiquitylase USP7. Here we unveil a functional cooperation between USP7 and VCP in the control of DNA replication conserved from Caenorhabditis elegans to mammals. The function of VCP during DNA replication is mediated by its cofactor FAF1, which facilitates the extraction of SUMOylated proteins that accumulate on chromatin upon USP7 inhibition. Supporting this cooperative role, the inactivation of the orthologues of USP7 and FAF1 is synthetic lethal in C. elegans, and USP7 and FAF1 inhibitors display synergistic toxicity. Together, these results reveal a coordinated activity of USP7 and VCPFAF1 in limiting SUMOylation at active replication forks and promoting the extraction and degradation of ubiquitylated proteins.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_05:29:18.616.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Eduardo Zarzuela
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	LTQ Orbitrap Velos

Revision	Datetime	Status	ChangeLog Entry
0	2020-04-17 05:33:35	ID requested
1	2021-09-12 03:42:51	announced
⏵ 2	2024-10-22 05:29:19	announced	2024-10-22: Updated project metadata.

Dataset with its publication pending

submitter keyword: Human, Crosslinking, VCP, DSP, USP7

Javier Muñoz
contact affiliation	CNIO
contact email	jmunozpe@cnio.es
lab head
Eduardo Zarzuela
contact affiliation	Centro Nacional de Investigaciones Oncológicas
contact email	eduzarfer@gmail.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/09/PXD018623

PRIDE project URI

• PRIDE
  1. PXD018623
     1. Label: PRIDE project
     2. Name: USP7 and VCPFAF1 define the SUMO/Ubiquitin landscape at DNA replication forks