PXD018604

PXD018604 is an original dataset announced via ProteomeXchange.

Title	Differences and commonalities in plasma membrane recruitment of the two morphogenetically distinct retroviruses HIV-1 and MMTV
Description	The targeting of retroviral Gag polyproteins to the inner leaflet of the plasma membrane is mediated by the N-terminal matrix (MA) domain of Gag. Since retroviruses of different morphogenetic types assemble their immature particles in distinct regions of the host cell, the mechanism of plasma membrane targeting differs between distinct retroviral morphogenetic types. To address this, we have focused on possible mechanistic differences of the MA-plasma membrane targeting of the B type mouse mammary tumor virus (MMTV) and C type HIV-1, which assembles in the cytoplasm and at the plasma membrane, respectively. Molecular dynamic (MD) simulations together with surface mapping have indicated that MMTV uses, similarly to HIV-1, the myristic switch mechanism to anchor the MA to the membrane and electrostatically interacts with phosphatidylinositol 4,5-bisphosphate to stabilize MA orientation. We have observed that the affinity of the MMTV MA to the membrane is lower than the affinity of the HIV 1 MA. This might be related to their different topology and the number of basic residues in the highly basic region, which probably reflects the requirement of C-type retroviruses for tighter membrane binding, essential for the assembly, in contrast to D/B-type retroviruses, assembling in the cytoplasm. Additionally, a comparison of the membrane topology of the HIV-1 MA using the surface mapping method and MD simulations has shown that the residues located at the C-terminus of the HIV-1 MA could be responsible for the stabilization of protein-protein interactions within the HIV-1 MA lattice at the plasma membrane.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_05:03:30.892.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD018604
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Petra Junková
SpeciesList	scientific name: Human immunodeficiency virus 1; NCBI TaxID: 11676; scientific name: Mouse mammary tumor virus; NCBI TaxID: 11757;
ModificationList	No PTMs are included in the dataset
Instrument	maXis

Revision	Datetime	Status	ChangeLog Entry
0	2020-04-16 08:49:19	ID requested
1	2020-05-11 18:34:25	announced
⏵ 2	2024-10-22 05:03:34	announced	2024-10-22: Updated project metadata.

10.1074/jbc.ra119.011991;

Junkov, á P, Pleskot R, Prchal J, S, ý, s J, Ruml T, Differences and commonalities in plasma membrane recruitment of the two morphogenetically distinct retroviruses HIV-1 and MMTV. J Biol Chem, 295(26):8819-8833(2020) [pubmed]

10.6019/PXD018604;

submitter keyword: HIV-1, covalent labeling-mass spectrometry, matrix protein, MMTV

Tomáš Ruml
contact affiliation	Department of Biochemistry and Microbiology, University of Chemistry and Technology, Prague, Technická 5, 166 28 Prague, Czech Republic
contact email	tomas.ruml@vscht.cz
lab head
Petra Junková
contact affiliation	Institute of Organic Chemistry and Biochemistry, Academy of Sciences of the Czech Republic, Flemingovo nám. 2, 166 10 Prague
contact email	petra.junkova@uochb.cas.cz
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD018604
     1. Label: PRIDE project
     2. Name: Differences and commonalities in plasma membrane recruitment of the two morphogenetically distinct retroviruses HIV-1 and MMTV