PXD018592

PXD018592 is an original dataset announced via ProteomeXchange.

Title	IL-22 promotes postnatal formation of MUC17 glycocalyx barrier that protects host enterocytes
Description	The apical aspects of absorptive epithelial cells form a highly organized brush border membrane (BBM), shaped by densely packed microvilli that are coated with a glycocalyx. Here, we present evidence showing that the glycocalyx forms an epithelial barrier that prevents exogenous molecules from gaining access to the BBM. We used a multiomics approach to investigate function and regulation of membrane mucins exposed on the enterocytic BBM during postnatal development of the small intestine. Muc17 was identified as a major membrane mucinin the glycocalyx that was specifically upregulated by IL-22 as part of an epithelial defense repertoire during suckling-weaning transition. High levels of IL-22 at the time of weaning primed progenitor enterocyte to express Muc17 as they migrate out of intestinal crypts to replace neonatal enterocytes. Our findings propose a role for membrane mucin Muc17 in epithelial barrier function in the small intestine.
HostingRepository	PRIDE
AnnounceDate	2021-02-16
AnnouncementXML	Submission_2021-09-28_03:50:59.855.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Liisa Arike
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2020-04-15 15:55:34	ID requested
1	2021-02-16 02:25:57	announced
⏵ 2	2021-09-28 03:51:00	announced	2021-09-28: Updated publication reference for PubMed record(s): 33596425.

Layunta E, J, ä, verfelt S, Dolan B, Arike L, Pelaseyed T, IL-22 promotes the formation of a MUC17 glycocalyx barrier in the postnatal small intestine during weaning. Cell Rep, 34(7):108757(2021) [pubmed]

submitter keyword: MUC13, MUC17, mucin, glycocalyx, microvilli, enterocyte, intestine, innate immunity, microbiota

Liisa Arike
contact affiliation	Mucin Biology Group, Proteomics Laboratory, Department of Medical Biochemistry and Cell Biology, University of Gothenburg
contact email	liisaarike@gmail.com
lab head
Liisa Arike
contact affiliation	PhD
contact email	liisaarike@gmail.com
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD018592
     1. Label: PRIDE project
     2. Name: IL-22 promotes postnatal formation of MUC17 glycocalyx barrier that protects host enterocytes