PXD018586

PXD018586 is an original dataset announced via ProteomeXchange.

Title	COMPARATIVE ANALYSIS OF THE PROTEOME OF KUPFFER CELLS AND MONOCYTES
Description	Macrophage population in most mammalian organs consists of cells of different origin, with the exception of the central nervous system and the liver, where macrophages of monocytic origin are almost completely absent. The reasons for such distribution and the phenomenon of coexistence of the two separate macrophage lineages with different origin in mammals remain poorly understood. In present study we compared Kupffer cells and monocytes by the immunophenotype, gene expression profile, proteome and pool of mircoRNA. Observed differences do not allow to consider the resident liver macrophages as purely M2 macrophages or monocytes have purely M1 features. Two populations of macrophages of monocytic origin and resident macrophages have fundamentally different roles in maintaining homeostasis. Monocytic macrophages are involved in the regulation of inflammation, and resident macrophages are involved in the regulation of specific organ functions (nitrogen metabolism, complement system protein synthesis). However, if other indicators would be considered as markers of macrophage activity, it is worth noting that Kupffer cells possess some features of M2 macrophages. This is indicated by their expression profile of let-7b/c/d/e miRNAs, a high content of proteins associated with oxidative phosphorylation, as well as an increased level of synthesis of Arg1, IL10.
HostingRepository	PRIDE
AnnounceDate	2021-09-09
AnnouncementXML	Submission_2021-09-08_18:32:07.934.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Sergey Kovalchuk
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	monohydroxylated residue; acetylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2020-04-15 15:52:06	ID requested
⏵ 1	2021-09-08 18:32:08	announced

Elchaninov A, Lokhonina A, Nikitina M, Vishnyakova P, Makarov A, Arutyunyan I, Poltavets A, Kananykhina E, Kovalchuk S, Karpulevich E, Bolshakova G, Sukhikh G, Fatkhudinov T, Comparative Analysis of the Transcriptome, Proteome, and miRNA Profile of Kupffer Cells and Monocytes. Biomedicines, 8(12):(2020) [pubmed]

submitter keyword: macrophages, monocytes, Kuppfer cells

Sergey I. Kovalchuk
contact affiliation	Shemyakin-Ovchinnikov Insitute of Bioorganic Chemistry, RAS, Moscow, Russia
contact email	xerx222@gmail.com
lab head
Sergey Kovalchuk
contact affiliation	Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Moscow, RAS
contact email	xerx222@gmail.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/09/PXD018586

PRIDE project URI

• PRIDE
  1. PXD018586
     1. Label: PRIDE project
     2. Name: COMPARATIVE ANALYSIS OF THE PROTEOME OF KUPFFER CELLS AND MONOCYTES