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PXD018585

PXD018585 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleProteins and Molecular Pathways Relevant for Stemness Properties of Tumor-Initiating Pancreatic Cancer Stem Cells Recognized by Differential Proteome-, Gene Expression-, and Functional Analysis
DescriptionCancer stem cells (CSCs), a small subset of the tumor bulk with highly malignant properties, are deemed responsible for tumor initiation, growth, metastasis, and relapse. In order to reveal molecular markers and determinants of stemness properties, pancreatic CSCs were enriched in three-dimensional spheroid cultures of two highly metastatic pancreatic cancer cell lines (L3.6sl and L3.6pl) and compared to bulk tumor cells using differential proteomics (PTX). We identified about 400 putative target proteins with significantly different expression in pancreatic CSCs and bulk tumor cells. By combining the unbiased PTX with gene expression analysis using quantitative polymerase chain reaction (qPCR) we nominated the two calcium binding proteins S100A8 (MRP8) and S100A9 (MRP14), as well as galactin-3-binding protein LGALS3BP (MAC-2-BP) as putative determinants of pancreatic CSCs. In silico pathway analysis followed by candidate-based RNA interference mediated functional analysis revealed a critical role of S100A8, S100A9, and LGALS3BP as molecular determinants of CSC proliferation, migration and in vivo tumor initiation. Our study highlights the power of combining unbiased proteomics with focused gene expression and functional analyses for the identification of key regulators of CSCs, an approach that warrants further application to identify CSCs proteins amenable to drug targeting.
HostingRepositoryPRIDE
AnnounceDate2024-10-22
AnnouncementXMLSubmission_2024-10-22_05:07:51.290.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterConstantin Blöchl
SpeciesList scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationListiodoacetamide derivatized residue
InstrumentLTQ Orbitrap XL
Dataset History
RevisionDatetimeStatusChangeLog Entry
02020-04-15 15:51:44ID requested
12020-06-25 01:24:05announced
22024-10-22 05:07:52announced2024-10-22: Updated project metadata.
Publication List
10.3390/cells9061397;
Samonig L, Loipetzberger A, Bl, ö, chl C, Rurik M, Kohlbacher O, Aberger F, Huber CG, Proteins and Molecular Pathways Relevant for the Malignant Properties of Tumor-Initiating Pancreatic Cancer Cells. Cells, 9(6):(2020) [pubmed]
Keyword List
submitter keyword: MRP14,proteomics, S100A9, MAC-2-BP, LGALS3BP, pancreatic cancer stem cells, S100A8, MRP8, S100 proteins, functional assays, differential proteome analysis, tumor grafting model
Contact List
Christian G. Huber
contact affiliationBioanalytical Research Labs, Department of Biosciences, University of Salzburg
contact emailc.huber@sbg.ac.at
lab head
Constantin Blöchl
contact affiliationDepartment of Biosciences, University of Salzburg
contact emailconstantin.bloechl@sbg.ac.at
dataset submitter
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Dataset FTP location
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PRIDE project URI
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