PXD018585

PXD018585 is an original dataset announced via ProteomeXchange.

Title	Proteins and Molecular Pathways Relevant for Stemness Properties of Tumor-Initiating Pancreatic Cancer Stem Cells Recognized by Differential Proteome-, Gene Expression-, and Functional Analysis
Description	Cancer stem cells (CSCs), a small subset of the tumor bulk with highly malignant properties, are deemed responsible for tumor initiation, growth, metastasis, and relapse. In order to reveal molecular markers and determinants of stemness properties, pancreatic CSCs were enriched in three-dimensional spheroid cultures of two highly metastatic pancreatic cancer cell lines (L3.6sl and L3.6pl) and compared to bulk tumor cells using differential proteomics (PTX). We identified about 400 putative target proteins with significantly different expression in pancreatic CSCs and bulk tumor cells. By combining the unbiased PTX with gene expression analysis using quantitative polymerase chain reaction (qPCR) we nominated the two calcium binding proteins S100A8 (MRP8) and S100A9 (MRP14), as well as galactin-3-binding protein LGALS3BP (MAC-2-BP) as putative determinants of pancreatic CSCs. In silico pathway analysis followed by candidate-based RNA interference mediated functional analysis revealed a critical role of S100A8, S100A9, and LGALS3BP as molecular determinants of CSC proliferation, migration and in vivo tumor initiation. Our study highlights the power of combining unbiased proteomics with focused gene expression and functional analyses for the identification of key regulators of CSCs, an approach that warrants further application to identify CSCs proteins amenable to drug targeting.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_05:07:51.290.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Constantin Blöchl
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	iodoacetamide derivatized residue
Instrument	LTQ Orbitrap XL

Revision	Datetime	Status	ChangeLog Entry
0	2020-04-15 15:51:44	ID requested
1	2020-06-25 01:24:05	announced
⏵ 2	2024-10-22 05:07:52	announced	2024-10-22: Updated project metadata.

10.3390/cells9061397;

Samonig L, Loipetzberger A, Bl, ö, chl C, Rurik M, Kohlbacher O, Aberger F, Huber CG, Proteins and Molecular Pathways Relevant for the Malignant Properties of Tumor-Initiating Pancreatic Cancer Cells. Cells, 9(6):(2020) [pubmed]

submitter keyword: MRP14,proteomics, S100A9, MAC-2-BP, LGALS3BP, pancreatic cancer stem cells, S100A8, MRP8, S100 proteins, functional assays, differential proteome analysis, tumor grafting model

Christian G. Huber
contact affiliation	Bioanalytical Research Labs, Department of Biosciences, University of Salzburg
contact email	c.huber@sbg.ac.at
lab head
Constantin Blöchl
contact affiliation	Department of Biosciences, University of Salzburg
contact email	constantin.bloechl@sbg.ac.at
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2020/06/PXD018585

PRIDE project URI

• PRIDE
  1. PXD018585
     1. Label: PRIDE project
     2. Name: Proteins and Molecular Pathways Relevant for Stemness Properties of Tumor-Initiating Pancreatic Cancer Stem Cells Recognized by Differential Proteome-, Gene Expression-, and Functional Analysis