PXD018584

PXD018584 is an original dataset announced via ProteomeXchange.

Title	The lncRNA Growth Arrest Specific 5 regulates cell survival via distinct structural modules with independent functions
Description	There is increasing evidence that the architecture of long non-coding RNAs – just like that of proteins – is hierarchically organized into independently folding sub-modules with distinct functions. Studies characterizing the cellular activities of such modules, however, are rare. The lncRNA growth arrest specific 5 (Gas5) is a key regulator of cell survival in response to stress and nutrient availability. We used SHAPE-MaP to probe the structure of Gas5 in vitro and in cellulo. The results show that Gas5 contains three separate structural modules including the previously predicted steroid receptor binding hairpin motif. Functional assays show that the newly identified modules act independently in leukemic T cells. The 5’ terminal module with low secondary structure content affects basal survival and slows the cell cycle, whereas the highly structured core module mediates the effects of mTOR inhibition on cell growth. Disruption of specific secondary structures within the modules abolish their function in cells. These results highlight the central role of Gas5 in regulating cell survival and reveals how a single lncRNA transcript utilizes a modular structure-function relationship to respond to a variety of cellular stresses under various cellular conditions.
HostingRepository	PRIDE
AnnounceDate	2021-04-07
AnnouncementXML	Submission_2021-04-06_20:08:48.576.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Duc Duong
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Fusion

Revision	Datetime	Status	ChangeLog Entry
0	2020-04-15 15:49:32	ID requested
⏵ 1	2021-04-06 20:08:49	announced

Frank F, Kavousi N, Bountali A, Dammer EB, Mourtada-Maarabouni M, Ortlund EA, The lncRNA Growth Arrest Specific 5 Regulates Cell Survival via Distinct Structural Modules with Independent Functions. Cell Rep, 32(3):107933(2020) [pubmed]

submitter keyword: Non-coding RNA, Proteomics, Growth Arrest

Eric Ortlund
contact affiliation	Professor Emory Biochemistry
contact email	eortlun@emory.edu
lab head
Duc Duong
contact affiliation	Center of Neurodegenerative Diseases
contact email	dduong@emory.edu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/04/PXD018584

PRIDE project URI

• PRIDE
  1. PXD018584
     1. Label: PRIDE project
     2. Name: The lncRNA Growth Arrest Specific 5 regulates cell survival via distinct structural modules with independent functions