PXD018576 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Essential PBP1-associated proteins of Trypanosoma brucei |
Description | Control of gene expression in kinetoplastids depends heavily on RNA-binding proteins that influence mRNA decay and translation. We previously showed that MKT1 interacts with PBP1, which in turn recruits LSM12 and poly(A) binding protein. MKT1 is recruited to mRNA by sequence-specific RNA-binding proteins, resulting in stabilisation of mRNA. We here show that PBP1, LSM12 and an additional 117-residue protein, XAC1 (Tb927.7.2780), are present in complexes that contain either MKT1 or MKT1L (Tb927.10.1490). All five proteins are present predominantly in the complexes, and there was evidence for a minor subset of complexes that contained both MKT1 and MKT1L. MKT1 appeared to be associated with many mRNAs, with the exception of those encoding ribosomal proteins. XAC1-containing complexes reproducibly contained RNAbinding proteins that were previously found associated with MKT1. In addition, however, XAC1- or MKT1- containing complexes specifically recruit one of the six translation initiation complexes, EIF4E6-EIF4G5; and yeast 2-hybrid assay results indicated that MKT1 interacts with EIF4G5. The C-terminus of MKT1L resembles MKT1: it contains MKT1 domains and a PIN domain that is probably not active as an endonuclease. MKT1L, however, also has an N-terminal extension with regions of low-complexity. Although MKT1L depletion inhibited cell proliferation, we found no evidence for specific interactions with RNA-binding proteins or mRNA. Deletion of the N-terminal extension, however, enabled MKT1L to interact with EIF4E6. We speculate that MKT1L may either enhance or inhibit the functions of MKT1-containing complexes. |
HostingRepository | PRIDE |
AnnounceDate | 2020-07-14 |
AnnouncementXML | Submission_2020-07-14_02:44:32.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Thomas Ruppert |
SpeciesList | scientific name: Trypanosoma brucei; NCBI TaxID: 5691; |
ModificationList | No PTMs are included in the dataset |
Instrument | LTQ Orbitrap Velos; LTQ Orbitrap |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2020-04-15 03:47:49 | ID requested | |
⏵ 1 | 2020-07-14 02:44:33 | announced | |
Publication List
Dataset with its publication pending |
Keyword List
submitter keyword: Trypanosoma, mRNA, MKT1, PBP1, Ataxin-2, poly(A) binding protein |
Contact List
Thomas Ruppert |
contact affiliation | Zentrum für Molekulare Biologie der University Heidelberg (ZMBH), Core Facility for mass spectrometry and proteomics, Im Neuenheimer Feld 282, 69120 Heid3elberg, Germany |
contact email | t.ruppert@zmbh.uni-heidelberg.de |
lab head | |
Thomas Ruppert |
contact affiliation | ZMBH, Im Neuenheimer Feld 282, 69122 Heidelberg |
contact email | t.ruppert@zmbh.uni-heidelberg.de |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2020/07/PXD018576 |
PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD018576
- Label: PRIDE project
- Name: Essential PBP1-associated proteins of Trypanosoma brucei