PXD018564

PXD018564 is an original dataset announced via ProteomeXchange.

Title	Fibrinogen function achieved through multiple covalent states
Description	Disulfide bonds link pairs of cysteine amino acids and their formation is assumed to be complete in the mature, functional protein. We tested this assumption by quantifying the redox state of disulfide bonds in the thrombosis protein, fibrinogen. There is an extraordinary disulfide lability in fibrinogen, with 13 bonds in the protein ranging from 10 to 50% reduced in human donors, indicating that fibrinogen exists in hundreds of different disulfide-bonded states. The significance of this finding for fibrinogen conversion to fibrin was investigated. Fibrin polymer formation triggers formation of disulfides in fibrin molecules, which is required for a robust fibrin matrix that withstands the mechanical forces of flowing blood and resists premature fibrinolysis. The covalent states of fibrinogen are changed by fluid shear forces ex vivo and in vivo, indicating that the different states are dynamic. These findings demonstrate that proteins can exist and function as a multitude of covalent forms.
HostingRepository	PRIDE
AnnounceDate	2020-11-09
AnnouncementXML	Submission_2020-11-09_07:43:08.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Diego Butera
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	iodoacetamide derivatized residue
Instrument	LTQ Orbitrap Velos

Revision	Datetime	Status	ChangeLog Entry
0	2020-04-15 00:50:48	ID requested
⏵ 1	2020-11-09 07:43:09	announced

Butera D, Hogg PJ, Fibrinogen function achieved through multiple covalent states. Nat Commun, 11(1):5468(2020) [pubmed]

submitter keyword: Fibrinogen, alpha2 macroglobulin,Cysteines, Redox state

Philip Hogg
contact affiliation	Head, ACRF Centenary Cancer Research Centre, The Charles Perkins Centre, The University of Sydney, NSW, Australia
contact email	phil.hogg@sydney.edu.au
lab head
Diego Butera
contact affiliation	ACRF Centenary Cancer Research Centre
contact email	diegobutera@gmail.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2020/11/PXD018564

PRIDE project URI

• PRIDE
  1. PXD018564
     1. Label: PRIDE project
     2. Name: Fibrinogen function achieved through multiple covalent states