PXD018563

PXD018563 is an original dataset announced via ProteomeXchange.

Title	Mitochondrial dysfunction caused by bacterial outer membranes vesicles activates intrinsic apoptosis and inflammation
Description	Sensing of microbes activates the immune system, depending on functional mitochondria. However, pathogenic bacteria inhibit mitochondria activity by delivering toxins via outer membrane vesicles (OMVs). How innate immune cells respond to pathogenic microbes that target mitochondria remains unclear. Here, we show that macrophages induce mitochondrial apoptosis and the NLRP3 inflammasome in response OMVs. Macrophages treated with OMVs and toxins that cause mitochondria dysfunction cease host protein translation which depletes pro-survival BCL-2 family member, MCL-1, and induces BAK-dependent mitochondrial apoptosis. Mitochondrial apoptosis and potassium ion efflux activate the NLRP3 inflammasome after OMV exposure. Importantly, mitochondrial apoptosis modulates IL-1 serum levels in response to OMVs. Our data highlight how innate immune cells sense infections by monitoring mitochondrial health.
HostingRepository	PRIDE
AnnounceDate	2020-07-30
AnnouncementXML	Submission_2020-08-22_16:44:27.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Cheng Huang
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Fusion

Revision	Datetime	Status	ChangeLog Entry
0	2020-04-15 00:50:31	ID requested
1	2020-07-30 00:54:32	announced
⏵ 2	2020-08-22 16:44:28	announced	2020-08-23: Updated publication reference for PubMed record(s): 32807891.

Deo P, Chow SH, Han ML, Speir M, Huang C, Schittenhelm RB, Dhital S, Emery J, Li J, Kile BT, Vince JE, Lawlor KE, Naderer T, Mitochondrial dysfunction caused by outer membrane vesicles from Gram-negative bacteria activates intrinsic apoptosis and inflammation. Nat Microbiol, 5(11):1418-1427(2020) [pubmed]

submitter keyword: macrophage, mitochondria, cell death, pyroptosis, apoptosis, toxin, OMV, inflammation, Neisseria, bacteria

Thomas Naderer
contact affiliation	Department of Biochemistry & Molecular Biology, Monash Biomedicine Discovery Institute, Monash University, Clayton 3800, Australia
contact email	thomas.naderer@monash.edu
lab head
Cheng Huang
contact affiliation	Monash University
contact email	cheng.huang@monash.edu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2020/07/PXD018563

PRIDE project URI

• PRIDE
  1. PXD018563
     1. Label: PRIDE project
     2. Name: Mitochondrial dysfunction caused by bacterial outer membranes vesicles activates intrinsic apoptosis and inflammation