PXD018558

PXD018558 is an original dataset announced via ProteomeXchange.

Title	Dual element (C/Cl) isotope analysis indicates distinct mechanisms of reductive dehalogenation of chlorinated ethenes and dichloroethane in Dehalococcoides mccartyi strain BTF08 with defined reductive dehalogenase inventories
Description	Dehalococcoides mccartyi strain BTF08 has the unique property to couple complete dechlorination of tetrachloroethene and 1,2-dichloroethane to ethene with growth by using the halogenated compounds as terminal electron acceptor. The genome of strain BTF08 encodes 20 genes for reductive dehalogenase homologous proteins (RdhA) including those described for dehalogenation of tetrachloroethene (PceA, PteA), trichloroethene (TceA) and vinyl chloride (VcrA). Thus far it is unknown under which conditions the different RdhAs are expressed, what their substrate specificity is and if different reaction mechanisms are employed. Here we found by proteomic analysis from differentially activated batches that PteA and VcrA were expressed during dechlorination of tetrachloroethene to ethene, while TceA was expressed during 1,2-dichloroethane dehalogenation. Carbon and chlorine compound-specific stable isotope analysis suggested distinct reaction mechanisms for the dechlorination of (i) cis-dichloroethene and vinyl chloride and (ii) tetrachloroethene. This differentiation was observed independent of the expressed RdhA proteins. Differently, two stable isotope fractionation patterns were observed for 1,2-dichloroethane transformation, for cells with distinct RdhA inventories. Conclusively, we could link specific RdhA expression with functions and provide an insight into the apparently substrate-specific reaction mechanisms in the pathway of reductive dehalogenation in D. mccartyi strain BTF08.
HostingRepository	PRIDE
AnnounceDate	2020-06-10
AnnouncementXML	Submission_2020-06-09_23:33:50.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Lorenz Adrian
SpeciesList	scientific name: Dehalococcoides mccartyi BTF08; NCBI TaxID: 1193806;
ModificationList	iodoacetamide derivatized residue
Instrument	Orbitrap Fusion

Revision	Datetime	Status	ChangeLog Entry
0	2020-04-14 23:51:28	ID requested
⏵ 1	2020-06-09 23:33:50	announced

Dataset with its publication pending

submitter keyword: Compound-specific stable isotope analysis, nLC-MS/MS, Dehalococcoides mccartyi strain BTF08, reductive dehalogenation, 1,2-dichloroethane, tetrachloroethene, cis-dichloroethene, vinyl chloride

Ivonne Nijenhuis
contact affiliation	Department Isotope Biogeochemistry, Helmholtz Centre for Environmental Research – UFZ, Permoserstrasse 15, 04107 Leipzig, Germany
contact email	Ivonne.nijenhuis@ufz.de
lab head
Lorenz Adrian
contact affiliation	Helmholtz Centre for Environmental Research - UFZ
contact email	lorenz.adrian@ufz.de
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD018558
     1. Label: PRIDE project
     2. Name: Dual element (C/Cl) isotope analysis indicates distinct mechanisms of reductive dehalogenation of chlorinated ethenes and dichloroethane in Dehalococcoides mccartyi strain BTF08 with defined reductive dehalogenase inventories