PXD018552

PXD018552 is an original dataset announced via ProteomeXchange.

Title	Analysis of CD5 interactome in primary mouse CD4+ T cells
Description	CD4+ T cells were isolated from gene-targeted mouse expressing a One-Strep-tag (OST) at the C terminus of the CD5 protein, briefly expanded in vitro and we analyzed the interactome of their OST-tagged CD5 protein. Affinity purification of the OST tagged protein was performed using Streptactin beads, from T cells either non-stimulated, stimulated 30s, 120s or 300s with anti-CD3 and anti-CD4 antibodies, or 300s with pervanadate. Each AP-MS purification is associated with a corresponding control (purification from CD4+ T cells isolated from WT mice, cultured and stimulated in the same conditions). The number of replicate biological experiments was n=3 for all conditions (time-points), and each sample was analyzed once by single-run nanoLC-MS, resulting in 30 raw files.
HostingRepository	PRIDE
AnnounceDate	2020-09-29
AnnouncementXML	Submission_2020-09-29_03:40:30.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Anne Gonzalez de Peredo
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	phosphorylated residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2020-04-14 07:57:00	ID requested
⏵ 1	2020-09-29 03:40:30	announced

Dataset with its publication pending

submitter keyword: TCR, interactome, signalosome, primary CD4+ T cell, AP-MS

Odile Burlet-Schiltz
contact affiliation	Institute of Pharmacology and Structurale Biology, CNRS, Toulouse
contact email	odile.schiltz@ipbs.fr
lab head
Anne Gonzalez de Peredo
contact affiliation	CNRS/IPBS
contact email	gonzalez@ipbs.fr
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2020/09/PXD018552

PRIDE project URI

• PRIDE
  1. PXD018552
     1. Label: PRIDE project
     2. Name: Analysis of CD5 interactome in primary mouse CD4+ T cells