PXD018545

PXD018545 is an original dataset announced via ProteomeXchange.

Title	Endoplasmic Reticulum Stress-mediated Mitochondrial Dysfunction in Aged Hearts
Description	Aging impairs the mitochondrial electron transport chain (ETC), especially in interfibrillar mitochondria (IFM). Mitochondria are in close contact with the endoplasmic reticulum (ER). Induction of ER stress leads to ETC injury in adult heart mitochondria. We asked if ER stress contributes to the mitochondrial dysfunction during aging. Subsarcolemmal mitochondria (SSM) and IFM were isolated from 3, 18, and 24 mo. C57Bl/6 mouse hearts. ER stress progressively increased with age, especially in 24 mo. mice that manifest mitochondrial dysfunction. OXPHOS was decreased in 24 mo. IFM oxidizing complex I and complex IV substrates. Proteomic analysis showed that the content of multiple complex I subunits was decreased in IFM from 24 mo. hearts, but remained unchanged in in 18 mo. IFM without a decrease in OXPHOS. Feeding mice with 4-phenylbutyrate (4-PBA) for two weeks attenuated the ER stress and improved mitochondrial function in 24 mo. mice. These results indicate that ER stress contributes to the mitochondrial dysfunction in aged hearts. Attenuation of ER stress is a potential approach to improve mitochondrial function in aged hearts.
HostingRepository	PRIDE
AnnounceDate	2020-07-23
AnnouncementXML	Submission_2020-07-23_06:40:54.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Ling Li
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2020-04-14 07:23:32	ID requested
⏵ 1	2020-07-23 06:40:55	announced

Chen Q, Samidurai A, Thompson J, Hu Y, Das A, Willard B, Lesnefsky EJ, Endoplasmic reticulum stress-mediated mitochondrial dysfunction in aged hearts. Biochim Biophys Acta Mol Basis Dis, 1866(11):165899(2020) [pubmed]

submitter keyword: Aging, ER stress, mitochondrial dysfunction

Edward Lesnefsky
contact affiliation	VCU Health, Department of Internal Medicine Division of Cardiology
contact email	edward.lesnefsky@vcuhealth.org
lab head
Ling Li
contact affiliation	Cleveland Clinic
contact email	lil5@ccf.org
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD018545
     1. Label: PRIDE project
     2. Name: Endoplasmic Reticulum Stress-mediated Mitochondrial Dysfunction in Aged Hearts