PXD018544
PXD018544 is an original dataset announced via ProteomeXchange.
Dataset Summary
| Title | Chemical proteomics profiling of N-terminal and lysine acetyltransferases |
| Description | Acetylation of amino groups is a prevalent protein modification in all kingdoms of life. Acetyl groups are transferred from Coenzyme A (CoA) to protein N-termini and lysine side chains by N-terminal acetyltransferases (NATs) and lysine acetyltransferases (KATs), respectively. Building on lysine-CoA conjugates as KAT probes, we have synthesized N-terminal CoA-conjugated peptide probes for interactome profiling of NAT complexes. These probes specifically recruited catalytic and auxiliary subunits of the major NAT complexes from cell lysates. When comparing the specificity of N-terminal and lysine CoA-conjugated probes the latter bound only a subset of endogenous KATs and appeared more prone to recruitment of other CoA-binding proteins. Western blot validation confirmed the specificity of selected NATs and KATs towards these probes supporting the notion that N-terminal CoA-conjugated peptides are versatile probes for NAT complex profiling in lysates of physiological and pathological backgrounds. |
| HostingRepository | jPOST |
| AnnounceDate | 2021-04-14 |
| AnnouncementXML | Submission_2023-02-07_00:05:04.501.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | Jürgen Eirich |
| SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
| ModificationList | S-carboxamidomethyl-L-cysteine; N6-acetyl-L-lysine; L-methionine sulfoxide |
| Instrument | Q Exactive |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|---|---|---|
| 0 | 2020-04-14 05:56:34 | ID requested | |
| 1 | 2021-04-13 08:00:05 | announced | |
| 2 | 2022-09-18 03:18:57 | announced | 2022-09-18: Updated FTP location. |
| ⏵ 3 | 2023-02-07 00:05:05 | announced | 2023-02-07: Updated PubMed. |
Publication List
| Sindlinger J, Sch, ö, n S, Eirich J, Kirchg, ä, ß, ner S, Finkemeier I, Schwarzer D, Investigating Peptide-Coenzyme A Conjugates as Chemical Probes for Proteomic Profiling of N-Terminal and Lysine Acetyltransferases. Chembiochem, 23(17):e202200255(2022) [pubmed] |
Keyword List
| submitter keyword: Acetylation, NAT, KAT, CoA |
Contact List
| Iris Finkemeier | |
|---|---|
| lab head | |
| Jürgen Eirich | |
| contact affiliation | University of Münster - WWU - Institute for Biology and Biotechnology of Plants |
| dataset submitter | |
Full Dataset Link List
| jPOST dataset URI |
| Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.jpostdb.org/JPST000827/ |
to receive all new ProteomeXchange dataset release announcements!
