PXD018530

PXD018530 is an original dataset announced via ProteomeXchange.

Title	The PII protein-protein interaction network in Azospirillum brasilense reveals a novel role of PII in the control of c-di-GMP homeostasis
Description	The PII family comprises a group of widely distributed signal transduction proteins ubiquitous present in prokaryotes and in the chloroplast of plants. The PII proteins sense the levels of key metabolites ATP, ADP and 2-oxoglutarate which affect the PII structure and the ability of PII to interact with a range of target proteins. Here we performed multiple PII ligand fishing assays to identify proteins that are likely to be part of the PII protein interaction network in plant-growth promoting nitrogen-fixing bacterium Azospirillum brasilense. Among the PII targets identified were enzymes related to nitrogen and fatty acid metabolism, signaling, co-enzyme synthesis, RNA catabolism and transcription. Direct binary PII-target complex was confirmed for xx protein complex using pull-down assays with recombinant proteins. Untargeted metabolome showed that PII is required to proper maintenance of Coenzyme A, panthotenate, PRPP and SAICAR levels. Two enzymes involved in c-di-GMP metabolism were among the identified PII targets. A PII minus strain showed reduced c-di-GMP levels and swimming behavior. These data support that PII acts as a major metabolic hub controlling key metabolic enzymes and c-di-GMP homeostasis accordingly to the prevailing nutritional status.
HostingRepository	PRIDE
AnnounceDate	2020-10-23
AnnouncementXML	Submission_2020-10-22_22:26:49.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Gustavo De Souza
SpeciesList	scientific name: Azospirillum brasilense Sp245; NCBI TaxID: 1064539;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2020-04-14 02:45:57	ID requested
⏵ 1	2020-10-22 22:26:49	announced

Dataset with its publication pending

submitter keyword: protein interaction, PII protein, c-di-GMP, motility, metabolic regulation

Luciano Huergo
contact affiliation	Departamento de Bioquímica e Biologia Molecular, UFPR Curitiba, PR, Brazil
contact email	luciano.huergo@gmail.com
lab head
Gustavo De Souza
contact affiliation	Dept. of Biochemistry, Federal University of Rio Grande do Norte
contact email	gadsouza@gmail.com
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD018530
     1. Label: PRIDE project
     2. Name: The PII protein-protein interaction network in Azospirillum brasilense reveals a novel role of PII in the control of c-di-GMP homeostasis