PXD018527

PXD018527 is an original dataset announced via ProteomeXchange.

Title	Analysis of the interactome of CD6 in CRISPR/CAS9 modified mouse CD4+ T cells
Description	Using a CRISPR/Cas9-based approach we engineer primary CD4+ T cells in which the CD6 protein was tagged with an affinity Twin-Strep-tag (OST) with the purpose of determining by quantitative mass spectrometry the composition and dynamics of the signalosome assembling around the tagged protein prior to and following T cell activation. Affinity purification of the OST tagged protein was performed using Streptactin beads, from T cells that were non-stimulated, or stimulated for 30s or 120s with anti-CD3 and anti-CD4 antibodies, or 300s with pervanadate. Each AP-MS purification is associated with a corresponding control (purification from non edited WT CD4+ T cells, cultured and stimulated in the same conditions). The number of replicate biological experiments was n=5 (cells non-stimulated or stimulated for 30s with anti-CD3/CD4), n=2 (cells stimulated for120s with antiCD3/CD4), or n=3 (cells stimulated 300s with pervanadate), and each sample was analyzed in duplicate or triplicate by LC-MS, resulting in 78 raw files.
HostingRepository	PRIDE
AnnounceDate	2020-09-29
AnnouncementXML	Submission_2020-09-29_03:44:17.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Anne Gonzalez de Peredo
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	phosphorylated residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2020-04-14 02:34:44	ID requested
⏵ 1	2020-09-29 03:44:18	announced

Dataset with its publication pending

submitter keyword: TCR, interactome, primary T cell, OST tag, CRISPR/CAS9, AP-MS

Odile Burlet-Schiltz
contact affiliation	Institute of Pharmacology and Structural Biology, CNRS, Toulouse
contact email	odile.schiltz@ipbs.fr
lab head
Anne Gonzalez de Peredo
contact affiliation	CNRS/IPBS
contact email	gonzalez@ipbs.fr
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2020/09/PXD018527

PRIDE project URI

• PRIDE
  1. PXD018527
     1. Label: PRIDE project
     2. Name: Analysis of the interactome of CD6 in CRISPR/CAS9 modified mouse CD4+ T cells