PXD018517

PXD018517 is an original dataset announced via ProteomeXchange.

Title	The RNF185/Membralin ubiquitin ligase complex is a new ERAD branch involved in the degradation of a distinct subset of integral membrane proteins.
Description	The endoplasmic reticulum-associated degradation (ERAD) pathway is responsible for ubiquitin-mediated quality control of secretory and ER-resident proteins. In the present study, two short-lived ER integral membrane model proteins were used to screen a genome-wide CRISPR-Cas9 library, resulting in the identification of a new ERAD branch consisting of the RNF185/Membralin ubiquitin ligase complex. Biochemical and affinity pull-down studies followed by mass spectrometry revealed that the ubiquitin-like domain containing proteins TMUB-1 and -2 are also part of the core complex being specifically enriched by RNF185/Membralin but not by other known ERAD factors like HRD1. Genetic studies showed that the RNF185/Membralin complex control the degradation of endogenous integral membrane proteins, including TMUB2 itself and CYP51A1 from which the initial recombinant model protein was derived.
HostingRepository	PRIDE
AnnounceDate	2024-10-07
AnnouncementXML	Submission_2024-10-07_10:39:21.691.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD018517
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Pedro Carvalho
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	Oxidation; Acetyl; acetylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2020-04-14 01:53:32	ID requested
⏵ 1	2024-10-07 10:39:22	announced

10.1016/j.molcel.2020.07.009;

van de Weijer ML, Krshnan L, Liberatori S, Guerrero EN, Robson-Tull J, Hahn L, Lebbink RJ, Wiertz EJHJ, Fischer R, Ebner D, Carvalho P, Quality Control of ER Membrane Proteins by the RNF185/Membralin Ubiquitin Ligase Complex. Mol Cell, 79(5):768-781.e7(2020) [pubmed]

10.6019/PXD018517;

submitter keyword: Endoplasmic reticulum-associated degradation (ERAD)

E3 ligase RNF185

Membralin

TMEM259

TMUB1

TMUB2

ubiquitin

Pedro Carvalho
contact affiliation	Sir William Dunn School of Pathology University of Oxford United Kingdom
contact email	pedro.carvalho@path.ox.ac.uk
lab head
Pedro Carvalho
contact affiliation	University of Oxford
contact email	pedro.carvalho@path.ox.ac.uk
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2020/08/PXD018517

PRIDE project URI

• PRIDE
  1. PXD018517
     1. Label: PRIDE project
     2. Name: The RNF185/Membralin ubiquitin ligase complex is a new ERAD branch involved in the degradation of a distinct subset of integral membrane proteins.