PXD018514

PXD018514 is an original dataset announced via ProteomeXchange.

Title	The Staphylococcus aureus Host Cell Genetic Adhesion Network
Description	Adhesion of Staphylococcus aureus to the host’s skin and mucosae enables asymptomatic colonization and the establishment of infections. This process is facilitated by cell wall-anchored (CWA) adhesins that are covalently attached to the bacterial cell wall and exhibit affinity for host ligands. To gain insight into the cellular factors contributing to adhesion, we developed a sensitive and robust assay to enable the large-scale profiling of S. aureus adhesion to host ligands. Using this assay, we profiled a sequence defined transposon mutant library of the community-associated methicillin-resistant S. aureus (CA-MRSA) isolate USA300, to identify mutant strains with attenuated adhesion to human derived keratin, fibronectin and fibrinogen. Overall, we identified 21 adhesion defective mutants and we used this information to construct ‘The S. aureus Genetic Adhesion Network’. Importantly, we identified a core gene set involved in adhesion to all three host ligands and we delineated unique genetic signatures. In addition, we demonstrate a central requirement for autolysin-mediated daughter cell separation in S. aureus CWA protein-mediated adhesion to host ligands, which contributes to virulence in murine skin and soft tissue infection. This comprehensive study will inform strategies to inhibit S. aureus host cell adhesion, potentially enabling the development of anti-adhesive therapeutics to complement traditional antibacterial chemotherapy.
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_08:51:00.139.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Jennifer Geddes-McAlister
SpeciesList	scientific name: Staphylococcus aureus; NCBI TaxID: 1280;
ModificationList	amidated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2020-04-14 01:53:10	ID requested
1	2023-03-04 10:47:28	announced
⏵ 2	2023-11-14 08:51:00	announced	2023-11-14: Updated project metadata.

Dataset with its publication pending

submitter keyword: Anti-adhesives

anti-virulence

Staphylococcus aureus

MRSA

antibiotic resistance

cell wall-anchored proteins

autolysin

adhesome

Jennifer Geddes-McAlister
contact affiliation	Molecular and Cellular Biology Department, University of Guelph, 50 Stone Rd. E., Guelph, ON, N1H 2W1
contact email	jgeddesm@uoguelph.ca
lab head
Jennifer Geddes-McAlister
contact affiliation	University of Guelph
contact email	jgeddesm@uoguelph.ca
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2023/03/PXD018514

PRIDE project URI

• PRIDE
  1. PXD018514
     1. Label: PRIDE project
     2. Name: The Staphylococcus aureus Host Cell Genetic Adhesion Network