PXD018455
PXD018455 is an original dataset announced via ProteomeXchange.
Dataset Summary
Title | Phosphoproteomic analysis reveals enhanced PKA signaling in aged skeletal muscle is suppressed by caloric restriction |
Description | Although caloric restriction (CR) was first shown to extend the lifespan of rodents over 75 years ago, the underlying mechanisms remain unclear. Additionally, the majority of published studies have focused on the effects of short-term CR. To better understand cell signaling alterations in a tissue known to be highly impacted by CR, we sought to assess the impact of aging and lifelong CR on skeletal muscle protein phosphorylation dynamics. We performed phosphoproteomic analysis on skeletal muscle from young mice, old mice fed on an ad libitum diet, and old mice fed a CR diet. This analysis revealed distinct phosphoproteomic signatures associated with both aging and diet. Importantly, CR appeared to promote a signature that was more similar to young mice than old mice fed on an ad lib diet. From the phosphorylation measurements on its known substrates, we deciphered that aging promotes Protein kinase A (PKA) signaling, and CR inhibits this signaling cascade. Given the demonstrated role of PKA signaling as a “pro-aging” pathway in various model organisms, including yeast and mice, we propose that CR exerts its longevity-enhancing effects partially via the suppression of this pathway. |
HostingRepository | jPOST |
AnnounceDate | 2021-01-30 |
AnnouncementXML | Submission_2024-05-09_11:32:47.617.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Paul Grimsrud |
SpeciesList | scientific name: Mus musculus (Mouse); NCBI TaxID: 10090; |
ModificationList | S-carboxamidomethyl-L-cysteine; O-phospho-L-serine; O-phospho-L-threonine; O4'-phospho-L-tyrosine; L-methionine sulfoxide; TMT6plex reporter+balance reagent N-acylated residue; TMT6plex reporter+balance reagent acylated N-terminal |
Instrument | Q Exactive |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
---|---|---|---|
0 | 2020-04-09 12:59:06 | ID requested | |
1 | 2021-01-30 11:23:57 | announced | |
2 | 2021-02-04 17:15:47 | announced | 2021-02-04: Updated FTP location. |
3 | 2022-09-18 04:02:07 | announced | 2022-09-18: Updated FTP location. |
⏵ 4 | 2024-05-09 11:32:48 | announced | 2024-05-10: Updated PubMed. |
Publication List
Bareja A, Draper JA, Katz LH, Lee DE, Grimsrud PA, White JP, Chronic caloric restriction maintains a youthful phosphoproteome in aged skeletal muscle. Mech Ageing Dev, 195():111443(2021) [pubmed] |
Keyword List
submitter keyword: aging |
caloric restriction |
phosphoproteome |
PKA |
mice |
Contact List
James P. White | |
---|---|
lab head | |
Paul Grimsrud | |
contact affiliation | Duke University |
dataset submitter |
Full Dataset Link List
jPOST dataset URI |
Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.jpostdb.org/JPST000789/ |