PXD018455

PXD018455 is an original dataset announced via ProteomeXchange.

Title	Phosphoproteomic analysis reveals enhanced PKA signaling in aged skeletal muscle is suppressed by caloric restriction
Description	Although caloric restriction (CR) was first shown to extend the lifespan of rodents over 75 years ago, the underlying mechanisms remain unclear. Additionally, the majority of published studies have focused on the effects of short-term CR. To better understand cell signaling alterations in a tissue known to be highly impacted by CR, we sought to assess the impact of aging and lifelong CR on skeletal muscle protein phosphorylation dynamics. We performed phosphoproteomic analysis on skeletal muscle from young mice, old mice fed on an ad libitum diet, and old mice fed a CR diet. This analysis revealed distinct phosphoproteomic signatures associated with both aging and diet. Importantly, CR appeared to promote a signature that was more similar to young mice than old mice fed on an ad lib diet. From the phosphorylation measurements on its known substrates, we deciphered that aging promotes Protein kinase A (PKA) signaling, and CR inhibits this signaling cascade. Given the demonstrated role of PKA signaling as a “pro-aging” pathway in various model organisms, including yeast and mice, we propose that CR exerts its longevity-enhancing effects partially via the suppression of this pathway.
HostingRepository	jPOST
AnnounceDate	2021-01-30
AnnouncementXML	Submission_2024-05-09_11:32:47.617.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Paul Grimsrud
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	S-carboxamidomethyl-L-cysteine; O-phospho-L-serine; O-phospho-L-threonine; O4'-phospho-L-tyrosine; L-methionine sulfoxide; TMT6plex reporter+balance reagent N-acylated residue; TMT6plex reporter+balance reagent acylated N-terminal
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2020-04-09 12:59:06	ID requested
1	2021-01-30 11:23:57	announced
2	2021-02-04 17:15:47	announced	2021-02-04: Updated FTP location.
3	2022-09-18 04:02:07	announced	2022-09-18: Updated FTP location.
⏵ 4	2024-05-09 11:32:48	announced	2024-05-10: Updated PubMed.

Bareja A, Draper JA, Katz LH, Lee DE, Grimsrud PA, White JP, Chronic caloric restriction maintains a youthful phosphoproteome in aged skeletal muscle. Mech Ageing Dev, 195():111443(2021) [pubmed]

submitter keyword: aging

caloric restriction

phosphoproteome

PKA

mice

James P. White
lab head
Paul Grimsrud
contact affiliation	Duke University
dataset submitter

jPOST dataset URI

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