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PXD018455

PXD018455 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitlePhosphoproteomic analysis reveals enhanced PKA signaling in aged skeletal muscle is suppressed by caloric restriction
DescriptionAlthough caloric restriction (CR) was first shown to extend the lifespan of rodents over 75 years ago, the underlying mechanisms remain unclear. Additionally, the majority of published studies have focused on the effects of short-term CR. To better understand cell signaling alterations in a tissue known to be highly impacted by CR, we sought to assess the impact of aging and lifelong CR on skeletal muscle protein phosphorylation dynamics. We performed phosphoproteomic analysis on skeletal muscle from young mice, old mice fed on an ad libitum diet, and old mice fed a CR diet. This analysis revealed distinct phosphoproteomic signatures associated with both aging and diet. Importantly, CR appeared to promote a signature that was more similar to young mice than old mice fed on an ad lib diet. From the phosphorylation measurements on its known substrates, we deciphered that aging promotes Protein kinase A (PKA) signaling, and CR inhibits this signaling cascade. Given the demonstrated role of PKA signaling as a “pro-aging” pathway in various model organisms, including yeast and mice, we propose that CR exerts its longevity-enhancing effects partially via the suppression of this pathway.
HostingRepositoryjPOST
AnnounceDate2021-01-30
AnnouncementXMLSubmission_2024-05-09_11:32:47.617.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterPaul Grimsrud
SpeciesList scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationListS-carboxamidomethyl-L-cysteine; O-phospho-L-serine; O-phospho-L-threonine; O4'-phospho-L-tyrosine; L-methionine sulfoxide; TMT6plex reporter+balance reagent N-acylated residue; TMT6plex reporter+balance reagent acylated N-terminal
InstrumentQ Exactive
Dataset History
RevisionDatetimeStatusChangeLog Entry
02020-04-09 12:59:06ID requested
12021-01-30 11:23:57announced
22021-02-04 17:15:47announced2021-02-04: Updated FTP location.
32022-09-18 04:02:07announced2022-09-18: Updated FTP location.
42024-05-09 11:32:48announced2024-05-10: Updated PubMed.
Publication List
Bareja A, Draper JA, Katz LH, Lee DE, Grimsrud PA, White JP, Chronic caloric restriction maintains a youthful phosphoproteome in aged skeletal muscle. Mech Ageing Dev, 195():111443(2021) [pubmed]
Keyword List
submitter keyword: aging
caloric restriction
phosphoproteome
PKA
mice
Contact List
James P. White
lab head
Paul Grimsrud
contact affiliationDuke University
dataset submitter
Full Dataset Link List
jPOST dataset URI
Dataset FTP location
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