PXD018451

PXD018451 is an original dataset announced via ProteomeXchange.

Title	PolyQ-expansion does not alter the huntingtin-HAP40 complex
Description	The amplification of a CAG repeat in the gene coding for huntingtin (HTT) leads to Huntington’s disease (HD). At the protein level, this translates into the expansion of a poly-glutamine (polyQ) stretch in the HTT N-terminus, which renders HTT aggregation-prone by unknown mechanisms. Here we investigate the effects of polyQ expansion on the HTT-HAP40 complex, where HTT structure is substantially stabilized. Surprisingly, our biophysical, cryo-EM and crosslinking mass spectrometry experiments reveal no major changes between 17QHTT-HAP40 (wild type), 46QHTT-HAP40 (typical polyQ length in HD patients) and 128QHTT-HAP40 (extreme polyQ length). Thus, the proposed destabilizing effect of HTT polyQ expansion may not suffice to alter the HTT-HAP40 complex, suggesting that polyQ expansion does not have a major global effect on HTT structure.
HostingRepository	PRIDE
AnnounceDate	2021-03-19
AnnouncementXML	Submission_2021-03-19_06:22:58.430.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Florian Stengel
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Fusion

Revision	Datetime	Status	ChangeLog Entry
0	2020-04-09 07:28:06	ID requested
⏵ 1	2021-03-19 06:22:58	announced

Dataset with its publication pending

submitter keyword: Huntingtin

Huntington’s disease

polyglutamine expansion

cryo-electron microscopy

crosslinking mass spectrometry

Florian Stengel
contact affiliation	Department of Biology, Cellular Proteostasis and Mass Sepectrometry, University of Konstanz
contact email	florian.stengel@uni-konstanz.de
lab head
Florian Stengel
contact affiliation	Universität Konstanz
contact email	florian.stengel@uni-konstanz.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/03/PXD018451

PRIDE project URI

• PRIDE
  1. PXD018451
     1. Label: PRIDE project
     2. Name: PolyQ-expansion does not alter the huntingtin-HAP40 complex