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PXD018450

PXD018450 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleProteomic profiling of HepG2 cell line after 2DE fractionation
DescriptionOne of the major goals of the Chromosome-centric Human Proteome Project (C-HPP) is to catalog and annotate myriads of heterogeneous proteoforms, produced by ca. 20 thousand genes. To achieve deeper and personalized dive into proteome we suggest to use customized RNA-seq library of potential proteoforms, which includes aberrant variants, specific to the certain biological sample. Multidimensional fractionation by means of two-dimensional electrophoresis and high-performance liquid chromatography, allowed us to downgrade the difficulty of biological mixture for the following shotgun mass spectrometry. To benchmark proposed pipeline we examined heterogeneity of the HepG2 hepatoblastome cell line proteome.
HostingRepositoryPRIDE
AnnounceDate2020-06-01
AnnouncementXMLSubmission_2020-06-01_14:52:15.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterOlga Kiseleva
SpeciesList scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationListmonohydroxylated residue; iodoacetamide derivatized residue
InstrumentQ Exactive
Dataset History
RevisionDatetimeStatusChangeLog Entry
02020-04-09 07:28:05ID requested
12020-06-01 14:52:16announced
Publication List
Kiseleva O, Ponomarenko E, Poverennaya E, Empowering Shotgun Mass Spectrometry with 2DE: A HepG2 Study. Int J Mol Sci, 21(11):(2020) [pubmed]
Keyword List
ProteomeXchange project tag: Human Proteome Project
submitter keyword: HepG2, proteoforms, LC-MS/MS
Contact List
Ekaterina Poverennaya
contact affiliationLab of proteoforms' interactomics, Institute of Biomedical Chemistry, Moscow, Russia
contact emailk.poverennaya@gmail.com
lab head
Olga Kiseleva
contact affiliationInstitute of Biomedical Chemistry
contact emailolly.kiseleva@gmail.com
dataset submitter
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Dataset FTP location
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PRIDE project URI
Repository Record List
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