PXD018450

PXD018450 is an original dataset announced via ProteomeXchange.

Title	Proteomic profiling of HepG2 cell line after 2DE fractionation
Description	One of the major goals of the Chromosome-centric Human Proteome Project (C-HPP) is to catalog and annotate myriads of heterogeneous proteoforms, produced by ca. 20 thousand genes. To achieve deeper and personalized dive into proteome we suggest to use customized RNA-seq library of potential proteoforms, which includes aberrant variants, specific to the certain biological sample. Multidimensional fractionation by means of two-dimensional electrophoresis and high-performance liquid chromatography, allowed us to downgrade the difficulty of biological mixture for the following shotgun mass spectrometry. To benchmark proposed pipeline we examined heterogeneity of the HepG2 hepatoblastome cell line proteome.
HostingRepository	PRIDE
AnnounceDate	2020-06-01
AnnouncementXML	Submission_2020-06-01_14:52:15.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Olga Kiseleva
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2020-04-09 07:28:05	ID requested
⏵ 1	2020-06-01 14:52:16	announced

Kiseleva O, Ponomarenko E, Poverennaya E, Empowering Shotgun Mass Spectrometry with 2DE: A HepG2 Study. Int J Mol Sci, 21(11):(2020) [pubmed]

ProteomeXchange project tag: Human Proteome Project

submitter keyword: HepG2, proteoforms, LC-MS/MS

Ekaterina Poverennaya
contact affiliation	Lab of proteoforms' interactomics, Institute of Biomedical Chemistry, Moscow, Russia
contact email	k.poverennaya@gmail.com
lab head
Olga Kiseleva
contact affiliation	Institute of Biomedical Chemistry
contact email	olly.kiseleva@gmail.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2020/06/PXD018450

PRIDE project URI

• PRIDE
  1. PXD018450
     1. Label: PRIDE project
     2. Name: Proteomic profiling of HepG2 cell line after 2DE fractionation