PXD018450 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Proteomic profiling of HepG2 cell line after 2DE fractionation |
Description | One of the major goals of the Chromosome-centric Human Proteome Project (C-HPP) is to catalog and annotate myriads of heterogeneous proteoforms, produced by ca. 20 thousand genes. To achieve deeper and personalized dive into proteome we suggest to use customized RNA-seq library of potential proteoforms, which includes aberrant variants, specific to the certain biological sample. Multidimensional fractionation by means of two-dimensional electrophoresis and high-performance liquid chromatography, allowed us to downgrade the difficulty of biological mixture for the following shotgun mass spectrometry. To benchmark proposed pipeline we examined heterogeneity of the HepG2 hepatoblastome cell line proteome. |
HostingRepository | PRIDE |
AnnounceDate | 2020-06-01 |
AnnouncementXML | Submission_2020-06-01_14:52:15.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Olga Kiseleva |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | monohydroxylated residue; iodoacetamide derivatized residue |
Instrument | Q Exactive |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2020-04-09 07:28:05 | ID requested | |
⏵ 1 | 2020-06-01 14:52:16 | announced | |
Publication List
Kiseleva O, Ponomarenko E, Poverennaya E, Empowering Shotgun Mass Spectrometry with 2DE: A HepG2 Study. Int J Mol Sci, 21(11):(2020) [pubmed] |
Keyword List
ProteomeXchange project tag: Human Proteome Project |
submitter keyword: HepG2, proteoforms, LC-MS/MS |
Contact List
Ekaterina Poverennaya |
contact affiliation | Lab of proteoforms' interactomics, Institute of Biomedical Chemistry, Moscow, Russia |
contact email | k.poverennaya@gmail.com |
lab head | |
Olga Kiseleva |
contact affiliation | Institute of Biomedical Chemistry |
contact email | olly.kiseleva@gmail.com |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD018450
- Label: PRIDE project
- Name: Proteomic profiling of HepG2 cell line after 2DE fractionation