PXD018449 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Phosphoproteomic analysis of signaling downstream of p85β |
Description | p85β is a regulatory subunit of phosphatidylinositol 3-kinase. The signaling mechanism of p85β is poorly understood. Quantitative mass spectrometry-based phosphoproteomic analysis was therefore performed to reveal proteins with an altered phosphorylation status upon PIK3R2 depletion in ovarian cancer cell line SKOV3. This profiling yielded 90 unique altered phosphopeptides, mapping to 29 and 45 proteins whose levels were downregulated or upregulated at P<0.05 by PIK3R2-specific siRNA, respectively. We observed significant enrichment of proteins in ubiquitin-mediated proteolysis and autophagy regulation, suggesting a role of p85β in regulating these cellular processes. |
HostingRepository | PRIDE |
AnnounceDate | 2024-10-22 |
AnnouncementXML | Submission_2024-10-22_05:03:23.463.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Lydia Cheung |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | phosphorylated residue |
Instrument | Orbitrap Fusion Lumos |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2020-04-09 07:28:05 | ID requested | |
1 | 2020-05-08 03:59:28 | announced | |
2 | 2020-05-12 22:18:02 | announced | 2020-05-13: Updated publication reference for PubMed record(s): 32385243. |
⏵ 3 | 2024-10-22 05:03:24 | announced | 2024-10-22: Updated project metadata. |
Publication List
Rao L, Mak VCY, Zhou Y, Zhang D, Li X, Fung CCY, Sharma R, Gu C, Lu Y, Tipoe GL, Cheung ANY, Mills GB, Cheung LWT, regulates autophagic degradation of AXL to activate oncogenic signaling. Nat Commun, 11(1):2291(2020) [pubmed] |
10.1038/s41467-020-16061-7; |
Keyword List
submitter keyword: Phosphoproteomics, PI3K, p85 |
Contact List
Dr CHEUNG, Lydia Wai Ting |
contact affiliation | Assistant Professor, School of Biomedical Sciences, Faculty of Medicine, The University of Hong Kong, HK SAR |
contact email | lydiacwt@hku.hk |
lab head | |
Lydia Cheung |
contact affiliation | University of Hong Kong |
contact email | lydiacwt@hku.hk |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD018449
- Label: PRIDE project
- Name: Phosphoproteomic analysis of signaling downstream of p85β