PXD018449

PXD018449 is an original dataset announced via ProteomeXchange.

Title	Phosphoproteomic analysis of signaling downstream of p85β
Description	p85β is a regulatory subunit of phosphatidylinositol 3-kinase. The signaling mechanism of p85β is poorly understood. Quantitative mass spectrometry-based phosphoproteomic analysis was therefore performed to reveal proteins with an altered phosphorylation status upon PIK3R2 depletion in ovarian cancer cell line SKOV3. This profiling yielded 90 unique altered phosphopeptides, mapping to 29 and 45 proteins whose levels were downregulated or upregulated at P<0.05 by PIK3R2-specific siRNA, respectively. We observed significant enrichment of proteins in ubiquitin-mediated proteolysis and autophagy regulation, suggesting a role of p85β in regulating these cellular processes.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_05:03:23.463.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Lydia Cheung
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	phosphorylated residue
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2020-04-09 07:28:05	ID requested
1	2020-05-08 03:59:28	announced
2	2020-05-12 22:18:02	announced	2020-05-13: Updated publication reference for PubMed record(s): 32385243.
⏵ 3	2024-10-22 05:03:24	announced	2024-10-22: Updated project metadata.

Rao L, Mak VCY, Zhou Y, Zhang D, Li X, Fung CCY, Sharma R, Gu C, Lu Y, Tipoe GL, Cheung ANY, Mills GB, Cheung LWT, regulates autophagic degradation of AXL to activate oncogenic signaling. Nat Commun, 11(1):2291(2020) [pubmed]

10.1038/s41467-020-16061-7;

submitter keyword: Phosphoproteomics, PI3K, p85

Dr CHEUNG, Lydia Wai Ting
contact affiliation	Assistant Professor, School of Biomedical Sciences, Faculty of Medicine, The University of Hong Kong, HK SAR
contact email	lydiacwt@hku.hk
lab head
Lydia Cheung
contact affiliation	University of Hong Kong
contact email	lydiacwt@hku.hk
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2020/05/PXD018449

PRIDE project URI

• PRIDE
  1. PXD018449
     1. Label: PRIDE project
     2. Name: Phosphoproteomic analysis of signaling downstream of p85β