PXD018444

PXD018444 is an original dataset announced via ProteomeXchange.

Title	TECAN Resolvex A200 Positive Pressure Workstation for Semi Automated Peptide Purification of Complex Proteomic Samples
Description	High-throughput has become a substantial need in many omics investigations. In proteomics the sample preparation workflow consists of multiple steps, whereby with each additional manual step more bias is added to the sample. Especially for label-free quantification experiments this drastically reduces the outcome. Here, a positive pressure workstation was evaluated to increase automation of sample preparation and decrease input time and consumables. The semi-automated workflow with the Resolvex A200 workstation including the sorbents Maestro and WWP2 performed equally well to the routinely conducted manual workflow, with similar technical variability in MSMS-based identifications of peptides and proteins. Both sorbents produced highly reproducible results within a range of 10-300 µg of peptide starting material. A direct, on-column sample preparation using the NBE columns - without conditioning and equilibration of the sorbents -works generally robust, whereby consumables and time are drastically reduced.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_05:18:05.898.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Louise Schelletter
SpeciesList	scientific name: Cricetulus griseus (Chinese hamster) (Cricetulus barabensis griseus); NCBI TaxID: 10029;
ModificationList	monohydroxylated residue; acetylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2020-04-09 04:20:01	ID requested
1	2021-03-04 13:22:59	announced
⏵ 2	2024-10-22 05:18:08	announced	2024-10-22: Updated project metadata.

10.1002/rcm.8873;

Schelletter L, Hertel O, Antar SJ, Scherling C, L, ä, ttig J, Noll T, Hoffrogge R, A positive pressure workstation for semi-automated peptide purification of complex proteomic samples. Rapid Commun Mass Spectrom, 35(2):e8873(2021) [pubmed]

submitter keyword: peptide purification,ppSPE, cell culture, fedbatch cultivation, high-throughput proteomics, sample preparation

Thomas Noll
contact affiliation	Cell Culture Technology, Technical Faculty, Bielefeld University, Germany
contact email	thomas.noll@uni-bielefeld.de
lab head
Louise Schelletter
contact affiliation	Bielefeld University
contact email	l.brachtvogel@uni-bielefeld.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/01/PXD018444

PRIDE project URI

• PRIDE
  1. PXD018444
     1. Label: PRIDE project
     2. Name: TECAN Resolvex A200 Positive Pressure Workstation for Semi Automated Peptide Purification of Complex Proteomic Samples