PXD018440

PXD018440 is an original dataset announced via ProteomeXchange.

Title	Proteomic analysis of human monosomic cell lines
Description	Chromosome loss that results in monosomy has detrimental consequences for human cells, but the underlying molecular mechanisms remain enigmatic. Using p53 deficient monosomic cell lines, we found that chromosome loss impairs proliferation and genomic stability. Transcriptome and proteome analysis revealed a partial compensation of the gene dosage changes that mitigates the effects of chromosome loss. Monosomy triggers global gene expression changes that differ from the effects of trisomy. Strikingly, ribosome biogenesis and translation were commonly downregulated in monosomic cells. Polysome profiling and translation analysis suggest that the defects arise due to haploinsufficiency of ribosomal genes. The ensuing ribosome biogenesis stress triggers the p53 pathway and G1 arrest when TP53 is reintroduced into monosomic cells. Accordingly, impaired ribosome biogenesis and p53 inactivation are associated with monosomy in cancer. Our first systematic study of monosomy in human cells demonstrates that haploinsufficiency of ribosomal genes presents a dominant negative phenotype of monosomy.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_05:23:02.891.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Paul Menges
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	TMT6plex-126 reporter+balance reagent acylated residue
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2020-04-09 01:12:41	ID requested
1	2021-06-17 23:57:45	announced
⏵ 2	2024-10-22 05:23:03	announced	2024-10-22: Updated project metadata.

Dataset with its publication pending

submitter keyword: TMT, Monosomy,Aneuploidy, LC-MS/MS

Prof. Dr. Zuzana Storchova
contact affiliation	Department of Biology, University Kaiserslautern, Germany.
contact email	storchova@bio.uni-kl.de
lab head
Paul Menges
contact affiliation	Technische Universität Kaiserslautern
contact email	pmenges@bio.uni-kl.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/06/PXD018440

PRIDE project URI

• PRIDE
  1. PXD018440
     1. Label: PRIDE project
     2. Name: Proteomic analysis of human monosomic cell lines