PXD018418

PXD018418 is an original dataset announced via ProteomeXchange.

Title	Essential functions of glycans in human epithelia dissected by a CRISPR/Cas9-engineered human organotypic skin model
Description	The glycome undergoes characteristic changes during histogenesis and organogenesis, but our understanding of the importance of select glycan structures for tissue formation and homeostasis is incomplete. Here, we present a human organotypic platform that allows genetic dissection of cellular glycosylation capacities and systematic interrogation of the roles of distinct glycan types in tissue formation. We used CRISPR-Cas9 gene targeting to generate a library of 3D organotypic skin tissues that selectively differ in their capacity to produce glycan structures on the main types of Nand O-linked glycoproteins and glycolipids. This tissue library revealed distinct changes in skin formation associated with a loss of features for all tested glycoconjugates. The organotypic skin model provides phenotypic cues for the distinct functions of glycoconjugates, and serves as a unique resource for further genetic dissection and identification of the specific structural features involved. The strategy is also applicable to other organotypic tissue models.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_05:27:47.487.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Sergey Vakhrushev
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	monohydroxylated residue
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2020-04-07 07:52:35	ID requested
1	2021-09-08 23:37:20	announced
⏵ 2	2024-10-22 05:27:54	announced	2024-10-22: Updated project metadata.

10.1016/j.devcel.2020.06.039;

Dabelsteen S, Pallesen EMH, Marinova IN, Nielsen MI, Adamopoulou M, R, ø, mer TB, Levann A, Andersen MM, Ye Z, Thein D, Bennett EP, B, ü, ll C, Moons SJ, Boltje T, Clausen H, Vakhrushev SY, Bagdonaite I, Wandall HH, Essential Functions of Glycans in Human Epithelia Dissected by a CRISPR-Cas9-Engineered Human Organotypic Skin Model. Dev Cell, 54(5):669-684.e7(2020) [pubmed]

submitter keyword: Skin, Glycoproteomics,Glycosylation

Sergey Vakhrushev
contact affiliation	Copenhagen Center for Glycomics, University of Copenhagen, Denmark
contact email	seva@sund.ku.dk
lab head
Sergey Vakhrushev
contact affiliation	Department of Cellular and Molecular Medicine
contact email	seva@sund.ku.dk
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/09/PXD018418

PRIDE project URI

• PRIDE
  1. PXD018418
     1. Label: PRIDE project
     2. Name: Essential functions of glycans in human epithelia dissected by a CRISPR/Cas9-engineered human organotypic skin model