PXD018412
PXD018412 is an original dataset announced via ProteomeXchange.
Dataset Summary
| Title | Identification of non-specific phosphatases in TCA cycle regulation |
| Description | Protein phosphorylation is a well-established post-translational mechanism that regulates protein functions and metabolic pathways. It has been shown that several plant mitochondrial proteins are phosphorylated in a reversible manner. However, the identity of the phosphatases/kinases involved in this mechanism and their role in the regulation of the TCA cycle remains unclear. Here, we isolated and characterized plants lacking two mitochondrially targeted phosphatases (Sal2 and PP2c63) alongside pyruvate dehydrogenase kinase (PDK). Protein-protein interaction, quantitative phosphoproteomics and enzymatic analyses revealed that PDK specifically regulates pyruvate dehydrogenase (PDH), whilst Sal2 and PP2c63 regulate PDHand fumarase. Alongside with the recombinant protein complemented the purified mitochondria, the PP2c63 directly regulate the PDH and fumarase while the Sal2 indirectly regulated. Characterization of steady-state metabolite levels and fluxes in the corresponding mutants revealed that these phosphatases regulate the fluxes through the TCA cycle, with altered metabolism compromising growth of the sal2/pp2c63 double. The combined data are collectively discussed in the context of current models of the control of respiration in plants. |
| HostingRepository | jPOST |
| AnnounceDate | 2021-04-01 |
| AnnouncementXML | Submission_2023-02-07_00:04:23.451.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | Jürgen Eirich |
| SpeciesList | scientific name: Arabidopsis thaliana (Mouse-ear cress); NCBI TaxID: 3702; |
| ModificationList | S-carboxamidomethyl-L-cysteine; alpha-amino acetylated residue; L-methionine sulfoxide; O-phospho-L-serine; O-phospho-L-threonine; O4'-phospho-L-tyrosine |
| Instrument | Q Exactive |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|---|---|---|
| 0 | 2020-04-07 00:41:18 | ID requested | |
| 1 | 2021-04-03 06:29:47 | announced | |
| 2 | 2022-09-18 02:25:45 | announced | 2022-09-18: Updated FTP location. |
| ⏵ 3 | 2023-02-07 00:04:24 | announced | 2023-02-07: Updated PubMed. |
Publication List
| Balparda M, Els, ä, sser M, Badia MB, Giese J, Bovdilova A, H, ü, dig M, Reinmuth L, Eirich J, Schwarzl, ä, nder M, Finkemeier I, Schallenberg-R, ü, dinger M, Maurino VG, Acetylation of conserved lysines fine-tunes mitochondrial malate dehydrogenase activity in land plants. Plant J, 109(1):92-111(2022) [pubmed] |
Keyword List
| submitter keyword: Arabidopsis thaliana, phosphoproteomics, regulation, plant, TCA cycle |
Contact List
| Iris Finkemeier | |
|---|---|
| lab head | |
| Jürgen Eirich | |
| contact affiliation | University of Münster - WWU - Institute for Biology and Biotechnology of Plants |
| dataset submitter | |
Full Dataset Link List
| jPOST dataset URI |
| Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.jpostdb.org/JPST000784/ |
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