PXD018405

PXD018405 is an original dataset announced via ProteomeXchange.

Title	Temporal proteomic profiling of Agrobacterium tumefaciens exposure to agroinfiltration media influences cellular remodeling and may promote enhanced adaptability for molecular farming
Description	Plants transformed via agroinfiltration using a modified strain of Agrobacterium tumefaciens can produce antibody proteins for purification and application in antibody-based therapeutics. The preparation of large quantities of plants for transformation may require bacterial exposure to agroinfiltration media, devoid of nutrients and carbon sources, for prolonged periods of time (e.g., 2 to 24 h). Such conditions may influence the viability of the bacteria and the subsequent levels of infection and target protein production. Here, we draw upon our previous work profiling growth-specific differences of A. tumefaciens associated with shake flask vs. bioreactor growth and explore the role of timing in bacterial culture preparation for agroinfiltration. We use state-of-the-art mass spectrometry-based proteomics to define processes of cellular remodeling over the time course of incubation in agroinfiltration media and detect proteins with significant changes in abundance. We observe distinct proteome profiles associated with bacterial growth conditions and exposure timing, as well as a progression towards increased cellular remodelling over time. In addition, we define unique functional characteristics influencing pathogenesis and response to stimulus, as well as changes in bacterial nutrient acquisition and adaptability. Moreover, we validate our findings with in-depth profiling of specific proteins over time and demonstrates an overlap >80% between our proteomics platforms. Overall, our results suggest that prolonged exposure of A. tumefaciens to agroinfiltration media may increase pathogenesis and motility, leading to enhanced infectivity of plant cells and production of the target drug.
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_08:50:57.245.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Jennifer Geddes-McAlister
SpeciesList	scientific name: Rhizobium radiobacter (Agrobacterium tumefaciens) (Agrobacterium radiobacter); NCBI TaxID: 358;
ModificationList	monohydroxylated residue; acetylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2020-04-06 07:29:52	ID requested
1	2020-12-10 09:51:29	announced
⏵ 2	2023-11-14 08:50:57	announced	2023-11-14: Updated project metadata.

Dataset with its publication pending

submitter keyword: trastuzumab, bioreactor,Agrobacterium tumefaciens, agroinfiltration media, shake flask, quantitative proteomics

Jennifer Geddes-McAlister
contact affiliation	Department of Molecular and Cellular Biology, University of Guelph, 50 Stone Rd. E., Guelph, Ontario, Canada, N1G 2W1
contact email	jgeddesm@uoguelph.ca
lab head
Jennifer Geddes-McAlister
contact affiliation	University of Guelph
contact email	jgeddesm@uoguelph.ca
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD018405
     1. Label: PRIDE project
     2. Name: Temporal proteomic profiling of Agrobacterium tumefaciens exposure to agroinfiltration media influences cellular remodeling and may promote enhanced adaptability for molecular farming