⮝ Full datasets listing

PXD018405

PXD018405 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleTemporal proteomic profiling of Agrobacterium tumefaciens exposure to agroinfiltration media influences cellular remodeling and may promote enhanced adaptability for molecular farming
DescriptionPlants transformed via agroinfiltration using a modified strain of Agrobacterium tumefaciens can produce antibody proteins for purification and application in antibody-based therapeutics. The preparation of large quantities of plants for transformation may require bacterial exposure to agroinfiltration media, devoid of nutrients and carbon sources, for prolonged periods of time (e.g., 2 to 24 h). Such conditions may influence the viability of the bacteria and the subsequent levels of infection and target protein production. Here, we draw upon our previous work profiling growth-specific differences of A. tumefaciens associated with shake flask vs. bioreactor growth and explore the role of timing in bacterial culture preparation for agroinfiltration. We use state-of-the-art mass spectrometry-based proteomics to define processes of cellular remodeling over the time course of incubation in agroinfiltration media and detect proteins with significant changes in abundance. We observe distinct proteome profiles associated with bacterial growth conditions and exposure timing, as well as a progression towards increased cellular remodelling over time. In addition, we define unique functional characteristics influencing pathogenesis and response to stimulus, as well as changes in bacterial nutrient acquisition and adaptability. Moreover, we validate our findings with in-depth profiling of specific proteins over time and demonstrates an overlap >80% between our proteomics platforms. Overall, our results suggest that prolonged exposure of A. tumefaciens to agroinfiltration media may increase pathogenesis and motility, leading to enhanced infectivity of plant cells and production of the target drug.
HostingRepositoryPRIDE
AnnounceDate2023-11-14
AnnouncementXMLSubmission_2023-11-14_08:50:57.245.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterJennifer Geddes-McAlister
SpeciesList scientific name: Rhizobium radiobacter (Agrobacterium tumefaciens) (Agrobacterium radiobacter); NCBI TaxID: 358;
ModificationListmonohydroxylated residue; acetylated residue; iodoacetamide derivatized residue
InstrumentOrbitrap Fusion Lumos
Dataset History
RevisionDatetimeStatusChangeLog Entry
02020-04-06 07:29:52ID requested
12020-12-10 09:51:29announced
22023-11-14 08:50:57announced2023-11-14: Updated project metadata.
Publication List
Dataset with its publication pending
Keyword List
submitter keyword: trastuzumab, bioreactor,Agrobacterium tumefaciens, agroinfiltration media, shake flask, quantitative proteomics
Contact List
Jennifer Geddes-McAlister
contact affiliationDepartment of Molecular and Cellular Biology, University of Guelph, 50 Stone Rd. E., Guelph, Ontario, Canada, N1G 2W1
contact emailjgeddesm@uoguelph.ca
lab head
Jennifer Geddes-McAlister
contact affiliationUniversity of Guelph
contact emailjgeddesm@uoguelph.ca
dataset submitter
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2020/12/PXD018405
PRIDE project URI
Repository Record List
[ + ]