PXD018403

PXD018403 is an original dataset announced via ProteomeXchange.

Title	Functional analysis of CNOT3 in pancreatic beta cells
Description	Pancreatic β-cells are responsible for production and secretion of insulin in response to increasing blood glucose levels. Therefore, defects in pancreatic β-cell function lead to hyperglycemia and diabetes mellitus. Understanding the molecular mechanisms governing β cell function is crucial for development of novel treatment strategies for this disease. The aim of this project was to investigate the role of Cnot3, part of CCR4-NOT complex, major deadenylase complex in mammals, in pancreatic β cell function. Cnot3βKO islets display decreased expression of key regulators of β cell maturation and function. Moreover, they show an increase of progenitor cell markers, β cell-disallowed genes and genes relevant to altered β cell function. Cnot3βKO islets exhibit altered deadenylation and increased mRNA stability, partly accounting for the increase of those genes. Together, these data reveal that CNOT3-mediated mRNA deadenylation and decay constitute previously unsuspected post-transcriptional mechanisms essential for β cell identity.
HostingRepository	PRIDE
AnnounceDate	2020-08-31
AnnouncementXML	Submission_2020-08-30_22:42:43.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Dina Mostafa
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2020-04-06 07:29:46	ID requested
⏵ 1	2020-08-30 22:42:43	announced

Mostafa D, Yanagiya A, Georgiadou E, Wu Y, Stylianides T, Rutter GA, Suzuki T, Yamamoto T, -cell identity and diabetic phenotype in mice caused by disruption of CNOT3-dependent mRNA deadenylation. Commun Biol, 3(1):476(2020) [pubmed]

submitter keyword: Mouse, islets

Tadashi Yamamoto
contact affiliation	Cell Signal unit, OIST Graduate University, Okinawa, Japan
contact email	tadashi.yamamoto@oist.jp
lab head
Dina Mostafa
contact affiliation	OIST Graduate University
contact email	dina.mostafa@oist.jp
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2020/08/PXD018403

PRIDE project URI

• PRIDE
  1. PXD018403
     1. Label: PRIDE project
     2. Name: Functional analysis of CNOT3 in pancreatic beta cells