PXD018392

PXD018392 is an original dataset announced via ProteomeXchange.

Title	Identification of differentially regulated proteins of Caenorhabditis elegans during Salmonella enterica Serovar Typhi exposure using Mass Spectrometry
Description	Recently, proteomics based studies have become the primary contributors for the understanding of host-pathogen interactions and discovery of various drug targets. Herewith, we have used the popular eukaryotic model organism Caenorhabditis elegans to study the host pathogen interactions at protein levels. Especially, we have employed proteomics approach to monitor the protein players that underwent for differential regulation during host-pathogen interaction through label-free quantitation LC-MS/MS technique. As a result of LC-MS/MS analysis, a total of 3747 differentially regulated proteins were identified during pathogenic condition among which 12 and 04 proteins were upregulated and downregulated with at least 2 folds, respectively. Bioinformatics analyses result suggested that the upregulated proteins have crucial roles in TCA cycle, whereas downregulated proteins were majorly involving in the N-glycosylation synthesis. Additionally, the mRNA levels of up and downregulated proteins were validated using qPCR analysis. Overall, the study suggested that the bacterial infection could target multiple molecular mechanisms to modulate the host machinery which favour the bacterial pathogen for their pathogenesis.
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_07:10:26.774.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Krishnaswamy Balamurugan
SpeciesList	scientific name: Caenorhabditis elegans; NCBI TaxID: 6239;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Synapt MS

Revision	Datetime	Status	ChangeLog Entry
0	2020-04-06 01:22:45	ID requested
1	2020-05-11 22:48:13	announced
2	2020-12-21 03:43:05	announced	2020-12-21: Updated publication reference for DOI(s): https://doi.org/10.1007/s42485-020-00033-y.
⏵ 3	2023-11-14 07:10:27	announced	2023-11-14: Updated project metadata.

Dataset with its publication pending

submitter keyword: LC-MS/MS, TCA cycle and qPCR, protein-protein interactions, gene ontology

Krishnaswamy Balamurugan
contact affiliation	Professor Department of Biotechnology Science Campus Alagappa University Karaikudi Tamil Nadu India
contact email	bsuryar@yahoo.com
lab head
Krishnaswamy Balamurugan
contact affiliation	Alagappa University
contact email	bsuryar@yahoo.com
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD018392
     1. Label: PRIDE project
     2. Name: Identification of differentially regulated proteins of Caenorhabditis elegans during Salmonella enterica Serovar Typhi exposure using Mass Spectrometry