PXD018386

PXD018386 is an original dataset announced via ProteomeXchange.

Title	Conditionally reprogrammed (CRC) and feeder free SMAD inhibited (SMADi) expanded human nasal epithelial cells label-free LC-MS/MS
Description	Discovery of therapies that are able to correct defective CFTR requires patient derived in vitro pre-clinical cell models to be developed. Two main approaches exist to expand bronchial and nasal cultures, such as conditionally reprogrammed cells (CRC) and feeder free dual SMAD inhibition (SMADi) to overcome senescence, low cell count, and increase passages. To mimic airway epithelium the expanded cells are differentiated at air liquid Interface (ALI). This project focused to compare the global proteome of ALI differentiated CRC and SMADi expanded HNECs both treated and untreated with CFTR corrector VX-809.
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_08:50:50.317.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Alexander Capraro
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	monomethylated residue; acetylated residue
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2020-04-06 01:21:53	ID requested
1	2021-12-05 14:30:53	announced
⏵ 2	2023-11-14 08:50:50	announced	2023-11-14: Updated project metadata.

Dataset with its publication pending

submitter keyword: human nasal epithelial cells,Cystic fibrosis, Conditionally reprogrammed cells, label-free LC-MS/MS, SMAD inhibition

Shafagh Waters
contact affiliation	Molecular and Integrative Cystic Fibrosis (miCF) Research Laboratory, School of Women’s and Children’s Health, UNSW Medicine, UNSW Sydney
contact email	shafagh.waters@unsw.edu.au
lab head
Alexander Capraro
contact affiliation	UNSW Australia
contact email	a.capraro@unsw.edu.au
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/12/PXD018386

PRIDE project URI

• PRIDE
  1. PXD018386
     1. Label: PRIDE project
     2. Name: Conditionally reprogrammed (CRC) and feeder free SMAD inhibited (SMADi) expanded human nasal epithelial cells label-free LC-MS/MS