PXD018384

PXD018384 is an original dataset announced via ProteomeXchange.

Title	Distinct proteome profiles of Agrobacterium tumefaciens grown in bioreactors suggests bacterial adaptability and preparedness for plant transformation in molecular farming
Description	Plants transformed via agroinfiltration using a modified strain of Agrobacterium tumefaciens can produce antibody proteins for purification and application in antibody-based therapeutics. The rising demand for such drugs underscores the expansion of molecular farming and warrants the need to produce transformed plants at an industrial scale, requiring large quantities of A. tumefaciens culture, which is not feasible using traditional growth methods (e.g., shake flask). To overcome this limitation, we investigate the use of a bioreactor growth chamber system to meet production demands through growth optimization and efficient plant transformation. We observe differences in target antibody production associated with different growth conditions and aim to characterize the unique responses through profiling of protein-level changes specific to each condition. Our quantitative proteomic profiling defines growth-specific alterations in protein abundance and highlights the functional roles of these proteins. Moreover, we observe changes in the bacterial proteomes, which may influence bacterial processes important for effective agroinfiltration and transformation. Overall, our study provides insight into fundamental biological processes of A. tumefaciens influenced by growth conditions, which may influence plant transformation efficiency and outcome of antibody-based therapeutic production.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_05:16:29.652.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Jennifer Geddes-McAlister
SpeciesList	scientific name: Rhizobium radiobacter (Agrobacterium tumefaciens) (Agrobacterium radiobacter); NCBI TaxID: 358;
ModificationList	monohydroxylated residue; acetylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2020-04-06 01:21:38	ID requested
1	2020-12-10 09:47:12	announced
⏵ 2	2024-10-22 05:16:30	announced	2024-10-22: Updated project metadata.

Dataset with its publication pending

submitter keyword: trastuzumab, bioreactor growth chamber,Agrobacterium tumefaciens, shake flask, quantitative proteomics

Jennifer Geddes-McAlister
contact affiliation	University of Guelph, Molecular and Cellular Biology Department, 50 Stone Rd. E., Guelph, Ontario, Canada, N1G 2W1
contact email	jgeddesm@uoguelph.ca
lab head
Jennifer Geddes-McAlister
contact affiliation	University of Guelph
contact email	jgeddesm@uoguelph.ca
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2020/12/PXD018384

PRIDE project URI

• PRIDE
  1. PXD018384
     1. Label: PRIDE project
     2. Name: Distinct proteome profiles of Agrobacterium tumefaciens grown in bioreactors suggests bacterial adaptability and preparedness for plant transformation in molecular farming