PXD018383

PXD018383 is an original dataset announced via ProteomeXchange.

Title	Proximal Biotinylation Based Combinatory Approach for Isolating Plasma Membrane Proteins
Description	Comprehensive profiling of the cell surface proteome has been challenging due to the lack of tools for an effective and reproducible way to isolate plasma membrane proteins from mammalian cells. Here, we employ a proximity-dependent biotinylation approach to label and isolate plasma membrane proteins without an extra in vitro labeling step that we called plasma membrane-BioID. The lipid modified BirA* enzyme (MyrPalm BirA*) was targeted to the plasma membrane where it effectively biotinylates plasma membrane proteins. Biotinylated proteins were then affinity purified and analyzed by mass spectrometry. Our analysis demonstrates that combining conventional sucrose density gradient centrifugation and plasma membrane-BioID is ideal to overcome the inherent limitations of identification of integral membrane proteins and yields highly pure plasma components for downstream proteomic analysis.
HostingRepository	PRIDE
AnnounceDate	2020-08-28
AnnouncementXML	Submission_2020-08-27_23:16:43.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Altuğ Kamacıoğlu
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	acetylated residue; monohydroxylated residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2020-04-06 01:21:12	ID requested
⏵ 1	2020-08-27 23:16:44	announced

Akdag M, Yunt ZS, Kamacioglu A, Qureshi MH, Akarlar BA, Ozlu N, Proximal Biotinylation-Based Combinatory Approach for Isolating Integral Plasma Membrane Proteins. J Proteome Res, 19(8):3583-3592(2020) [pubmed]

submitter keyword: cell surface, biotinylation, plasma membrane, sucrose density fractionation

Nurhan Ozlu
contact affiliation	Department of Molecular Biology and Genetics, Koc University, Istanbul, Turkey
contact email	nozlu@ku.edu.tr
lab head
Altuğ Kamacıoğlu
contact affiliation	Koç University
contact email	akamacioglu18@ku.edu.tr
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2020/08/PXD018383

PRIDE project URI

• PRIDE
  1. PXD018383
     1. Label: PRIDE project
     2. Name: Proximal Biotinylation Based Combinatory Approach for Isolating Plasma Membrane Proteins