PXD018374

PXD018374 is an original dataset announced via ProteomeXchange.

Title	Tandem affinity purification of monobodies against STAT3
Description	The aim of the proteomics experiment was to determine the specificity of the generated monobodies for the target protein STAT3. This was done in an affinity-enrichment experiment using two endogenously expressed and tandem-affinity-tagged monobodies in two different cell lines (Jurkat and K562). Eluted proteins after affinity purification were resolved by SDS-PAGE, and analysed using bottom-up proteomics workflow including tryptic digestion, LC-MS/MS, database-search and spectral counting.
HostingRepository	PRIDE
AnnounceDate	2020-07-10
AnnouncementXML	Submission_2020-08-20_23:45:10.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD018374
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Grégory La Sala
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	Ammonia-loss; Phospho; Dehydrated; Oxidation; Acetyl; Carbamidomethyl; Gln->pyro-Glu
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2020-04-04 15:47:17	ID requested
1	2020-07-10 03:38:18	announced
⏵ 2	2020-08-20 23:45:10	announced	2020-08-21: Updated publication reference for PubMed record(s): 32807795.

La Sala G, Michiels C, K, ü, kensh, ö, ner T, Brandstoetter T, Maurer B, Koide A, Lau K, Pojer F, Koide S, Sexl V, Dumoutier L, Hantschel O, Selective inhibition of STAT3 signaling using monobodies targeting the coiled-coil and N-terminal domains. Nat Commun, 11(1):4115(2020) [pubmed]

ProteomeXchange project tag: Cancer (B/D-HPP), Biology/Disease-Driven Human Proteome Project (B/D-HPP), Human Proteome Project

submitter keyword: STAT3, LC-MS/MS, monobody, TAP,

Oliver Hantschel
contact affiliation	Institute of Physiological Chemistry, Faculty of Medicine, Philipps-University of Marburg, Karl-von-Frisch-Straße 1, 35032 Marburg, Germany.
contact email	oliver.hantschel@uni-marburg.de
lab head
Grégory La Sala
contact affiliation	Swiss Institute for Experimental Cancer Research (ISREC), School of Life Sciences, École polytechnique fédérale de Lausanne (EPFL), Station 19, 1015 Lausanne, Switzerland.
contact email	gregory.lasala@epfl.ch
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2020/07/PXD018374

PRIDE project URI

• PRIDE
  1. PXD018374
     1. Label: PRIDE project
     2. Name: Tandem affinity purification of monobodies against STAT3