PXD018373

PXD018373 is an original dataset announced via ProteomeXchange.

Title	Conserved protein Pir2ARS2 mediates gene repression through cryptic introns in lncRNAs
Description	Long non-coding RNAs (lncRNAs) are components of epigenetic control mechanisms that ensure appropriate and timely gene expression. The functions of lncRNAs are often mediated through associated gene regulatory activities, but how lncRNAs are distinguished from other RNAs and recruit effector complexes is unclear. Here we utilize the fission yeast Schizosaccharomyces pombe to investigate how lncRNAs engage silencing activities to regulate gene expression in cis. We find that invasion of lncRNA transcription into the downstream gene body incorporates a cryptic intron required for repression of that gene. Our analyses show that lncRNAs containing cryptic introns are targeted by the conserved Pir2ARS2 protein in association with splicing factors, which recruit RNA processing and chromatin modifying activities involved in gene silencing. Pir2 and splicing machinery are broadly required for gene repression. Our finding that human ARS2 also interacts with splicing factors suggests a conserved mechanism mediates gene repression through cryptic introns within lncRNAs.
HostingRepository	PRIDE
AnnounceDate	2020-04-07
AnnouncementXML	Submission_2020-05-21_23:29:35.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD018373
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Lisa Jenkins
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	Oxidation; Acetyl; Carbamidomethyl
Instrument	Orbitrap Fusion

Revision	Datetime	Status	ChangeLog Entry
0	2020-04-04 10:18:27	ID requested
1	2020-04-06 22:46:17	announced
⏵ 2	2020-05-21 23:29:36	announced	2020-05-22: Updated publication reference for PubMed record(s): 32415063.

Thillainadesan G, Xiao H, Holla S, Dhakshnamoorthy J, Jenkins LMM, Wheeler D, Grewal SIS, mediates gene repression through cryptic introns in lncRNAs. Nat Commun, 11(1):2412(2020) [pubmed]

submitter keyword: RIME, lncRNA, ARS2

Lisa M. Jenkins
contact affiliation	Laboratory of Cell Biology, Center for Cancer Research, National Cancer Institute, NIH
contact email	lisa.jenkins@nih.gov
lab head
Lisa Jenkins
contact affiliation	National Cancer Institute, NIH
contact email	lisa.jenkins@nih.gov
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD018373
     1. Label: PRIDE project
     2. Name: Conserved protein Pir2ARS2 mediates gene repression through cryptic introns in lncRNAs