PXD018369

PXD018369 is an original dataset announced via ProteomeXchange.

Title	Exploration of human cerebrospinal fluid: A large proteome dataset revealed by trapped ion mobility time-of-flight mass spectrometry
Description	Cerebrospinal fluid (CSF) is a biofluid in direct contact with the brain and as such constitutes a sample of choice in the study of neurological disorders, including neurodegenerative diseases such as Alzheimer or Parkinson. Human CSF has still been less studied using proteomic technologies compared to other biological fluids such as blood plasma or serum. In this work, a pool of “normal” human CSF samples was analysed using a shotgun proteomic workflow that combined removal of highly abundant proteins by immunoaffinity depletion and isoelectric focussing electrophoresis fractionation of tryptic peptides to alleviate the complexity of the biofluid. The resulting 24 fractions were analysed using liquid chromatography coupled to a high-resolution and high-accuracy timsTOF Pro mass spectrometry instrument. This state-of-the-art protocol allowed the identification of 3’174 proteins in CSF.
HostingRepository	PRIDE
AnnounceDate	2020-05-07
AnnouncementXML	Submission_2020-07-22_06:40:12.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD018369
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Charlotte Macron
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	Ammonia-loss; Deamidated; TMT6plex; Dehydrated; Oxidation; Acetyl; Carbamidomethyl
Instrument	timsTOF Pro

Revision	Datetime	Status	ChangeLog Entry
0	2020-04-04 09:59:21	ID requested
1	2020-05-07 02:18:58	announced
⏵ 2	2020-07-22 06:40:13	announced	2020-07-22: Updated publication reference for PubMed record(s): 32478154.

Macron C, Lavigne R, N, ú, ñ, ez Galindo A, Affolter M, Pineau C, Dayon L, Exploration of human cerebrospinal fluid: A large proteome dataset revealed by trapped ion mobility time-of-flight mass spectrometry. Data Brief, 31():105704(2020) [pubmed]

submitter keyword: cerebrospinal fluid, large-scale proteome, mass spectrometry, LC-MS/MS, proteomics

Affolter Michael
contact affiliation	Proteomics group leader, Nestlé Institute for Food Safety & Analytical Sciences, Nestlé Research, Switzerland
contact email	michael.affolter@rdls.nestle.com
lab head
Charlotte Macron
contact affiliation	NIHS
contact email	charlotte.macron@rd.nestle.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2020/05/PXD018369

PRIDE project URI

• PRIDE
  1. PXD018369
     1. Label: PRIDE project
     2. Name: Exploration of human cerebrospinal fluid: A large proteome dataset revealed by trapped ion mobility time-of-flight mass spectrometry