PXD018364

PXD018364 is an original dataset announced via ProteomeXchange.

Title	Single or combined dietary supply of Psidium guajava and Phyllanthus amarus extracts differentially modulate immune responses and liver proteome in striped catfish (Pangasianodon hyphophthalmus)
Description	Guava Psidium guajava L (Pg) and bhumi amla Phyllanthus amarus Schum. et Thonn (Pa) are well-known plants in traditional medicine. However, the capacity of these plants for improving the immune system of aquatic species has received less attention so far. This study aimed to investigate the effects of single supply or mixture of Pg and Pa extracts on immune responses, disease resistance and liver proteome profiles in striped catfish Pangasianodon hypophthalmus. Fish were fed diets including basal diet 0% or one of three doses of each plant extract, either alone or in mixture, 0.08, 0.2 or 0.5% Pg, Pa or mixture (Pg:Pa, v/v) for 6 weeks. The immune parameters (respiratory burst activity (RBA); nitric oxide synthase (NOS), total immunoglobulin, lysozyme and complement activities) were examined at W3, W6 post-feeding, and after challenge test. The growth parameters and the challenge test with Edwardsiella ictaluri were done at W6. The liver proteome profiles were analysed in W6 at 0.08% and 0.5% of each extract. The results showed that extract-based diets significantly improved growth parameters in the Pg0.2 group compared to control. The cellular immune responses in spleen and the humoral immune responses in plasma were significantly improved in a dose and time-dependent manner. Diets supplemented with single Pg and Pa extracts, and to lesser extent to combined extracts, could significantly decrease the mortality of striped catfish following bacterial infection compared to control. The proteomic results indicated that some pathways related to immune responses, antioxidant and lipid metabolism were enriched in liver at W6. Several proteins (i.e., CD8B, HSP90AA1, HSP90AB1, PDIA3, CASP8, TUBA1C, CCKAR, GNAS, GRIN2D, PLCG1, PRKCA, SLC25A5, VDAC2, ACTN4, GNAI2, LCK, CARD9, NLRP12, and NLRP3) were synergistically upregulated in mixture of Pg and Pa-based diets compared to control and single dietary treatments. Taken together, the results revealed that single Pg and Pa extracts at 0.2 and 0.5% and their mixture at 0.08 and 0.5% have the potential to modulate the immune mechanisms and disease resistance of striped catfish. Moreover, the combination of Pg and Pa in diets suggested positive synergistic effects liver proteome profile related to immune system processes.
HostingRepository	PRIDE
AnnounceDate	2020-04-09
AnnouncementXML	Submission_2020-05-29_15:52:31.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Valérie Cornet
SpeciesList	scientific name: Pangasianodon hypophthalmus; NCBI TaxID: 310915;
ModificationList	S-carboxamidoethyl-L-cysteine; monohydroxylated residue
Instrument	Synapt MS

Revision	Datetime	Status	ChangeLog Entry
0	2020-04-04 09:25:11	ID requested
1	2020-04-09 03:03:18	announced
⏵ 2	2020-05-29 15:52:32	announced	2020-05-29: Updated publication reference for PubMed record(s): 32431710.

Nhu TQ, Bich Hang BT, Cornet V, Oger M, Bach LT, Anh Dao NL, Thanh Huong DT, Quetin-Leclercq J, Scippo ML, Phuong NT, Kestemont P, ). Front Immunol, 11():797(2020) [pubmed]

submitter keyword: Liver proteome profile, humoral immune response, Edwardsiella ictaluri, plant extract-based diets, Phyllanthus amarus Schum et Thonn, Psidium guajava L., skin mucosal immune response, striped catfish.

Patrick Kestemont
contact affiliation	Research Unit in Environmental and Evolutionary Biology (URBE), Institute of Life, Earth & Environment, University of Namur (UNamur), 5000 Namur, Belgium
contact email	patrick.kestemont@unamur.be
lab head
Valérie Cornet
contact affiliation	University of Namur
contact email	valerie.cornet@unamur.be
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD018364
     1. Label: PRIDE project
     2. Name: Single or combined dietary supply of Psidium guajava and Phyllanthus amarus extracts differentially modulate immune responses and liver proteome in striped catfish (Pangasianodon hyphophthalmus)