PXD018350

PXD018350 is an original dataset announced via ProteomeXchange.

Title	Trnp1 is a novel regulator of several nuclear membrane-less compartments
Description	TMF1-regulated nuclear protein 1 (Trnp1) has been shown to exert potent roles in neural development affecting neural stem cell self-renewal and brain folding, but its molecular function in the nucleus is still unknown. Here we show that Trnp1 is a low complexity protein with the capacity to phase separate. Trnp1 interacts with factors located in several nuclear membrane-less organelles, the nucleolus, nuclear speckles and condensed chromatin. Importantly, Trnp1 co-regulates the architecture and function of these nuclear compartments in vitro and in the developing brain in vivo. Deletion of a highly conserved region in the N-terminal intrinsic disordered region abolishes the capacity of Trnp1 to regulate nucleolar size and dynamics, proliferation and M-phase length, decreases the capacity to phase separate and abrogates most of Trnp1 protein interactions. Thus, we identified Trnp1 as a novel regulator of several nuclear membrane-less compartments, a function important to maintain cells in a self-renewing proliferative state.
HostingRepository	PRIDE
AnnounceDate	2020-08-25
AnnouncementXML	Submission_2020-08-25_06:20:06.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Ignasi Forne
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive HF; LTQ Orbitrap

Revision	Datetime	Status	ChangeLog Entry
0	2020-04-03 03:15:42	ID requested
⏵ 1	2020-08-25 06:20:07	announced

Esgleas M, Falk S, Forn, é I, Thiry M, Najas S, Zhang S, Mas-Sanchez A, Geerlof A, Niessing D, Wang Z, Imhof A, G, ö, tz M, Trnp1 organizes diverse nuclear membrane-less compartments in neural stem cells. EMBO J, 39(16):e103373(2020) [pubmed]

submitter keyword: Trnp1, Nucleoli, nuclear speckles, heterochromatin, phase transition

Prof. Dr. Magdalena Götz
contact affiliation	1-Physiological Genomics, Biomedical Center (BMC), Ludwig-Maximilians Universitaet Muenchen, 82152 Planegg/Munich, Germany. 2- Institute for Stem Cell Research, Helmholtz Zentrum Muenchen, German Research Center for Environmental Health, 85764 Neuherberg, Germany.
contact email	Magdalena.goetz@helmholtz-muenchen.de
lab head
Ignasi Forne
contact affiliation	Biomedical Center-LMU
contact email	ignasi.forne@lrz.uni-muenchen.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2020/08/PXD018350

PRIDE project URI

• PRIDE
  1. PXD018350
     1. Label: PRIDE project
     2. Name: Trnp1 is a novel regulator of several nuclear membrane-less compartments