PXD018348

PXD018348 is an original dataset announced via ProteomeXchange.

Title	Identification and characterization of the heat-induced plastidial stress granules-like foci in Arabidopsis thaliana.
Description	Plant cells, similar to animal cells repress translation under stressful conditions which leads to formation of cytosolic stress granules (SGs). Here, we report that in addition to cytosolic SGs plants also have plastidial SGs (pSGs). In order isolate and determine protein and metabolite composition of the pSG a combination of differential centrifugation and affinity purification methods was used. Plastidial Snowy Cotyledon 1 (SCO1) fused with GFP was used as a marker protein and its subcellular localization was confirmed by confocal microscope in a control as well as stress conditions. Multiple criteria were used to define pSG‐associated proteins. As the pSGs were only present in the heat/dark treated seedlings, we first selected for proteins that were either (i) respectively, present and absent in the pSGs isolate from the stress‐treated versus control seedlings, or (ii) significantly enriched (fold change > 2; t-test<0.05) in the SGs isolate from the stress‐treated versus the control seedlings. We then excluded cytosolic, nuclear, mitochondrial and membrane proteins, as these are unlikely to localize into plastidial SGs and can therefore be viewed as false positives. As an additional negative control, we compered obtained list of pSGs proteins with results gotten from independently performed experiment where we used seedlings over-expressing plastidial glyceraldehyde-3-phosphate dehydrogenase GAPCP1-GFP. In the absence of stress, GAPCP1-GFP aggregates into a dot-like condensate, which could be enriched and subsequently isolated using our protocol, but is clearly distinguishable from the SCO1-GFP pSGs. Interestingly, identified components of pSG are very similar to the ones from cytosolic SGs suggesting that the mechanism of their formation and function might be very similar.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_05:12:36.662.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Ewelina Sokolowska
SpeciesList	scientific name: Arabidopsis thaliana (Mouse-ear cress); NCBI TaxID: 3702;
ModificationList	iodoacetamide derivatized residue
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2020-04-03 03:15:39	ID requested
1	2020-09-24 01:40:17	announced
2	2020-11-24 22:44:41	announced	2020-11-25: Updated publication reference for PubMed record(s): 33224174.
⏵ 3	2024-10-22 05:12:37	announced	2024-10-22: Updated project metadata.

10.3389/fpls.2020.595792;

Chodasiewicz M, Sokolowska EM, Nelson-Dittrich AC, Masiuk A, Beltran JCM, Nelson ADL, Skirycz A, Stress Response. Front Plant Sci, 11():595792(2020) [pubmed]

submitter keyword: Stress granules , Arabidopsis thaliana, Co-IP, Interactions,AP_MS, Snowy Cotyledon 1. Stress treatment

Aleksandra Skirycz
contact affiliation	Small Molecules Signaling, Max Planck Institute of Molecular Plant Physiology Wissenschaftspark Potsdam-Golm, Germany
contact email	skirycz@mpimp-golm.mpg.de
lab head
Ewelina Sokolowska
contact affiliation	Max Planck Institute of Molecular Plant Physiology Wissenschaftspark Am Mühlenberg 1 14476 Potsdam-Golm
contact email	esokolowska@mpimp-golm.mpg.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2020/09/PXD018348

PRIDE project URI

• PRIDE
  1. PXD018348
     1. Label: PRIDE project
     2. Name: Identification and characterization of the heat-induced plastidial stress granules-like foci in Arabidopsis thaliana.