PXD018330

PXD018330 is an original dataset announced via ProteomeXchange.

Title	A mass spectrometry analysis of the TAK1 protein complex in Jurkat T cells.
Description	Negative regulation of immunoreceptor signaling is required for preventing hyperimmune activation and maintaining immune homeostasis. The roles of p38IP in immunoreceptor signaling remain unclear. Here, we show that p38IP suppresses T cell receptor (TCR)/LPS-activated NF-κB and p38 by targeting TAK1 kinase and that p38IP protein levels are downregulated in human-PBMCs from rheumatoid arthritis (RA) patients, inversely correlating with the enhanced activity of NF-κB and p38. Mechanistically, p38IP interacts with TAK1 to disassemble the TAK1-TAB (TAK1-binding protein) complex. p38IP overexpression decreases TCR-induced binding of K63-linked polyubiquitin (polyUb) chains to TAK1 but increases that to TAB2, and p38IP knockdown shows the opposite effects, indicating unanchored K63-linked polyUb chain transfer from TAB2 to TAK1. p38IP dynamically interacts with TAK1 upon stimulation, because of the higher binding affinity of TAK1 and p38IP for sequential polyUb binding by TAB2 and TAK1, respectively. Moreover, p38IP specifically scaffolds the deubiquitinase USP4 to deubiquitinate TAK1 once TAK1 is activated. These findings reveal a novel role and the mechanisms of p38IP in controlling TCR/LPS signaling and suggest that p38IP might participate in RA pathogenesis.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_05:03:05.593.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	chensi zhao
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	LTQ Orbitrap Elite

Revision	Datetime	Status	ChangeLog Entry
0	2020-04-01 23:05:01	ID requested
1	2020-04-30 03:14:27	announced
2	2020-07-22 06:02:35	announced	2020-07-22: Updated publication reference for PubMed record(s): 32410369.
⏵ 3	2024-10-22 05:03:06	announced	2024-10-22: Updated project metadata.

Wang XD, Zhao CS, Wang QL, Zeng Q, Feng XZ, Li L, Chen ZL, Gong Y, Han J, Li Y, The p38-interacting protein p38IP suppresses TCR and LPS signaling by targeting TAK1. EMBO Rep, 21(7):e48035(2020) [pubmed]

10.15252/embr.201948035;

submitter keyword: TAK1,human, Jurkat T cells

Yingqiu Li
contact affiliation	MOE Key Laboratory of Gene Function and Regulation, State Key Laboratory of Biocontrol, School of Life Sciences, Sun Yat-sen University, Guangzhou, China.
contact email	lsslyq@mail.sysu.edu.cn
lab head
chensi zhao
contact affiliation	Immunity
contact email	zhaochensi2009@163.com
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD018330
     1. Label: PRIDE project
     2. Name: A mass spectrometry analysis of the TAK1 protein complex in Jurkat T cells.