PXD018330 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | A mass spectrometry analysis of the TAK1 protein complex in Jurkat T cells. |
Description | Negative regulation of immunoreceptor signaling is required for preventing hyperimmune activation and maintaining immune homeostasis. The roles of p38IP in immunoreceptor signaling remain unclear. Here, we show that p38IP suppresses T cell receptor (TCR)/LPS-activated NF-κB and p38 by targeting TAK1 kinase and that p38IP protein levels are downregulated in human-PBMCs from rheumatoid arthritis (RA) patients, inversely correlating with the enhanced activity of NF-κB and p38. Mechanistically, p38IP interacts with TAK1 to disassemble the TAK1-TAB (TAK1-binding protein) complex. p38IP overexpression decreases TCR-induced binding of K63-linked polyubiquitin (polyUb) chains to TAK1 but increases that to TAB2, and p38IP knockdown shows the opposite effects, indicating unanchored K63-linked polyUb chain transfer from TAB2 to TAK1. p38IP dynamically interacts with TAK1 upon stimulation, because of the higher binding affinity of TAK1 and p38IP for sequential polyUb binding by TAB2 and TAK1, respectively. Moreover, p38IP specifically scaffolds the deubiquitinase USP4 to deubiquitinate TAK1 once TAK1 is activated. These findings reveal a novel role and the mechanisms of p38IP in controlling TCR/LPS signaling and suggest that p38IP might participate in RA pathogenesis. |
HostingRepository | PRIDE |
AnnounceDate | 2024-10-22 |
AnnouncementXML | Submission_2024-10-22_05:03:05.593.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | chensi zhao |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | No PTMs are included in the dataset |
Instrument | LTQ Orbitrap Elite |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2020-04-01 23:05:01 | ID requested | |
1 | 2020-04-30 03:14:27 | announced | |
2 | 2020-07-22 06:02:35 | announced | 2020-07-22: Updated publication reference for PubMed record(s): 32410369. |
⏵ 3 | 2024-10-22 05:03:06 | announced | 2024-10-22: Updated project metadata. |
Publication List
Wang XD, Zhao CS, Wang QL, Zeng Q, Feng XZ, Li L, Chen ZL, Gong Y, Han J, Li Y, The p38-interacting protein p38IP suppresses TCR and LPS signaling by targeting TAK1. EMBO Rep, 21(7):e48035(2020) [pubmed] |
10.15252/embr.201948035; |
Keyword List
submitter keyword: TAK1,human, Jurkat T cells |
Contact List
Yingqiu Li |
contact affiliation | MOE Key Laboratory of Gene Function and Regulation, State Key Laboratory of Biocontrol, School of Life Sciences, Sun Yat-sen University, Guangzhou, China. |
contact email | lsslyq@mail.sysu.edu.cn |
lab head | |
chensi zhao |
contact affiliation | Immunity |
contact email | zhaochensi2009@163.com |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD018330
- Label: PRIDE project
- Name: A mass spectrometry analysis of the TAK1 protein complex in Jurkat T cells.