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PXD018325

PXD018325 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleMammalian Fibroblast Turnover Rates, Protein Abundance, and Protein Damage Response
DescriptionWithin a cell, proteins are in a dynamic state of turnover and are continuously synthesized and degraded. As an energetically expensive cellular process, protein turnover can have two opposing effects on maintaining a healthy proteome during the lifespan of an organism. Rapid protein turnover can replace old and damaged proteins with newly synthesized proteins. However, the high energetic demands of this process can potentially generate damaging reactive oxygen species that comprise the long-term health of the proteome. Thus, the relationship between aging, protein turnover kinetics and energetic demands of an organism remain unclear. Here, we used a proteomic approach to measure global rates of protein turnover within cultured fibroblasts isolated from a number of species with a wide range of lifespans. We show that organismal lifespan is negatively correlated with global rates of turnover. By further comparing cells from mice and naked mole rats (a short-lived and long-lived rodent species, respectively) we show that the latter has slower rates of turnover, lower levels of ATP production and reduced cellular ROS levels. Despite its slower rate of protein turnover, naked mole rat cells are able to tolerate protein misfolding stress more effectively than mouse cells. We suggest that in lieu of rapid constitutive protein turnover, long-lived species such as the naked mole rat have may have evolved more energetically efficient mechanisms for selective clearance of damaged proteins.
HostingRepositoryPRIDE
AnnounceDate2024-10-22
AnnouncementXMLSubmission_2024-10-22_05:03:17.244.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterKyle Swovick
SpeciesList scientific name: Bos taurus (Bovine); NCBI TaxID: 9913; scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationListmonohydroxylated residue; acetylated residue; iodoacetamide derivatized residue
InstrumentOrbitrap Fusion Lumos; LTQ Orbitrap
Dataset History
RevisionDatetimeStatusChangeLog Entry
02020-04-01 08:35:08ID requested
12020-05-07 02:33:58announced
22024-10-22 05:03:18announced2024-10-22: Updated project metadata.
Publication List
10.1074/mcp.ra120.002301;
Keyword List
submitter keyword: Mammalian, SILAC, LFQ, Primary, iBAQ,Fibroblast
Contact List
Sina Ghaemmaghami
contact affiliationUniversity of Rochester Department of Biology, University of Rochester Medical Center Mass Spectrometry Resource Facility
contact emailsina.ghaemmaghami@rochester.edu
lab head
Kyle Swovick
contact affiliationUniversity of Rochester Medical Center Mass Spectrometry Shared Resource Laboratory
contact emailkswovick@ur.rochester.edu
dataset submitter
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Dataset FTP location
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