PXD018325

PXD018325 is an original dataset announced via ProteomeXchange.

Title	Mammalian Fibroblast Turnover Rates, Protein Abundance, and Protein Damage Response
Description	Within a cell, proteins are in a dynamic state of turnover and are continuously synthesized and degraded. As an energetically expensive cellular process, protein turnover can have two opposing effects on maintaining a healthy proteome during the lifespan of an organism. Rapid protein turnover can replace old and damaged proteins with newly synthesized proteins. However, the high energetic demands of this process can potentially generate damaging reactive oxygen species that comprise the long-term health of the proteome. Thus, the relationship between aging, protein turnover kinetics and energetic demands of an organism remain unclear. Here, we used a proteomic approach to measure global rates of protein turnover within cultured fibroblasts isolated from a number of species with a wide range of lifespans. We show that organismal lifespan is negatively correlated with global rates of turnover. By further comparing cells from mice and naked mole rats (a short-lived and long-lived rodent species, respectively) we show that the latter has slower rates of turnover, lower levels of ATP production and reduced cellular ROS levels. Despite its slower rate of protein turnover, naked mole rat cells are able to tolerate protein misfolding stress more effectively than mouse cells. We suggest that in lieu of rapid constitutive protein turnover, long-lived species such as the naked mole rat have may have evolved more energetically efficient mechanisms for selective clearance of damaged proteins.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_05:03:17.244.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Kyle Swovick
SpeciesList	scientific name: Bos taurus (Bovine); NCBI TaxID: 9913; scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	monohydroxylated residue; acetylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Fusion Lumos; LTQ Orbitrap

Revision	Datetime	Status	ChangeLog Entry
0	2020-04-01 08:35:08	ID requested
1	2020-05-07 02:33:58	announced
⏵ 2	2024-10-22 05:03:18	announced	2024-10-22: Updated project metadata.

10.1074/mcp.ra120.002301;

submitter keyword: Mammalian, SILAC, LFQ, Primary, iBAQ,Fibroblast

Sina Ghaemmaghami
contact affiliation	University of Rochester Department of Biology, University of Rochester Medical Center Mass Spectrometry Resource Facility
contact email	sina.ghaemmaghami@rochester.edu
lab head
Kyle Swovick
contact affiliation	University of Rochester Medical Center Mass Spectrometry Shared Resource Laboratory
contact email	kswovick@ur.rochester.edu
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD018325
     1. Label: PRIDE project
     2. Name: Mammalian Fibroblast Turnover Rates, Protein Abundance, and Protein Damage Response