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PXD018322

PXD018322 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleThe Bloom Syndrome Complex Senses RPA-Coated Single-Stranded DNA to Restart Stalled Replication Forks
DescriptionThe Bloom syndrome helicase BLM interacts with topoisomerase IIIa (TOP3A), RMI1 and RMI2 to form the BTR complex, which dissolves double Holliday junctions to produce non-crossover products during homologous recombination (HR). BLM also promotes DNA-end resection and stalled replication fork restart. However, it is still unclear how the activities of the BTR complex are regulated in cells. Here, we identify multiple highly conserved short linear peptide motifs within the BTR complex that interact cooperatively with RPA. Furthermore, we demonstrate that RPA-binding is essential for stable recruitment of BLM to DNA damage sites and for stalled fork restart, but not for its roles in HR. Disruption of RPA-binding thus constitutes a novel separation-of-function mutation of the BTR complex, and suggest a model in which BTR contains the intrinsic ability to measure levels of RPA-ssDNA at replication forks, which controls its recruitment and activation in response to replication stress.
HostingRepositoryPRIDE
AnnounceDate2024-10-07
AnnouncementXMLSubmission_2024-10-07_10:38:49.954.xml
DigitalObjectIdentifierhttps://dx.doi.org/10.6019/PXD018322
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportSupported dataset by repository
PrimarySubmitteriolanda Vendrell
SpeciesList scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationListOxidation
InstrumentQ Exactive
Dataset History
RevisionDatetimeStatusChangeLog Entry
02020-04-01 08:11:05ID requested
12024-10-07 10:38:50announced
Publication List
10.6019/PXD018322;
Keyword List
submitter keyword: Bloom syndrome,DNA damage response, BLM, RPA, homologous recombination, replication stress
Contact List
Andrew N. Blackford
contact affiliation1.MRC Weatherall Institute of Molecular Medicine, University of Oxford, John Radcliffe Hospital, Oxford OX3 9DS, UK 2.CRUK/MRC Oxford Institute for Radiation Oncology, Department of Oncology, University of Oxford, Oxford OX3 7DQ, UK
contact emailandrew.blackford@imm.ox.ac.uk
lab head
iolanda Vendrell
contact affiliationCRUK-MRC Oxford Institute for Radiation Oncology, Oxford University
contact emailiolanda.vendrell@oncology.ox.ac.uk
dataset submitter
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Dataset FTP location
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